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関連する概念動画

Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
Translational Regulation01:29

Translational Regulation

273
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
273
Stringent Response in E. coli01:23

Stringent Response in E. coli

92
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
92
Leaky Scanning02:28

Leaky Scanning

5.3K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K
Improving Translational Accuracy02:07

Improving Translational Accuracy

12.0K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
12.0K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

3.5K
3.5K

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関連する実験動画

Updated: Oct 21, 2025

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
10:15

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Published on: July 6, 2012

16.5K

単細胞リボセクでは,細胞周期に依存するトランスレーションの停止が示される.

Michael VanInsberghe1, Jeroen van den Berg2, Amanda Andersson-Rolf2

  • 1Oncode Institute, Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Utrecht, The Netherlands. m.vaninsberghe@hubrecht.eu.

Nature
|September 9, 2021
PubMed
まとめ
この要約は機械生成です。

研究者たちは 敏感な単細胞リボソームプロファイリング方法を開発しました この技術は,機械学習と統合され,細胞間変換と変異を研究するためにコドン解像度を達成します.

さらに関連する動画

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.6K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

11.6K

関連する実験動画

Last Updated: Oct 21, 2025

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
10:15

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Published on: July 6, 2012

16.5K
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.6K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

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科学分野:

  • 分子生物学
  • ゲノミクス
  • 細胞生物学

背景:

  • 単細胞配列化により ゲノム,エピゲノム,トランスクリプトームの分析が進んでいます
  • 単細胞レベルで翻訳を測定することは依然として大きな課題です.
  • 既存のタンパク質検出方法では,単細胞解像度が不足しています.

研究 の 目的:

  • 単細胞におけるリボソームのプロファイリングのための非常に敏感な方法を開発する.
  • 細胞翻訳を分析するために単一コードンの解像度を達成します.
  • 細胞の多様性におけるトランスレーションの役割を調査する.

主な方法:

  • リボソームプロファイリングプロトコルの強化により 感度が向上します
  • 単一コードンの解像度のための機械学習との統合.
  • 希少な細胞タイプを含む様々な細胞環境での検証

主要な成果:

  • 特定のコドンでリボソームが一時停止するアミノ酸の制限が示されています.
  • 細胞サイクルに依存するリボソームの停止が観察された.
  • ミトーシス中の GAA コドン停止が検出されました.
  • 稀な小腸内分泌細胞に この技術を適用した

結論:

  • 開発された技術は,単細胞翻訳の敏感で高解像度の分析を可能にします.
  • リボソームの停止は 細胞サイクルやミトーシスなどの 特定の細胞状態と関連しています
  • この方法は希少な細胞集団に適用でき,新しい研究の道を開きます.
  • 細胞多様性への翻訳的貢献を理解するための基本的なツールを提供します.