光を駆動する分子モーターは,アルカリ金属イオンの選択的輸送をフォスフォリピドビレイヤーを通して促進する

Wen-Zhi Wang¹, Li-Bo Huang², Shao-Ping Zheng²

¹SAMS Research Group, Institut Charles Sadron UPR22, Centre National de la Recherce Scientifique, Université de Strasbourg, 67000 Strasbourg, France.

Journal of the American Chemical Society

|September 14, 2021

まとめ

研究者は人工イオンチャネルを作るためライト駆動の回転モーターを開発しました光の活性化によりアルカリイオン輸送が 400%まで増加しました

科学分野:

超分子化学
膜生物物理学
ナノテクノロジー

背景:

人工チャネルは膜輸送の理解と操作に不可欠です
ナノスケールプロセスに対する精密な外部制御を提供します
18-クラウン-6のようなマクロサイクルは,選択的なイオン結合で知られています.

研究の目的:

光に反応する分子構造を選択的なイオン輸送のために設計する
脂質二層の間のイオン転移の光誘発加速のメカニズムを調査する.
分子モーターを用いた機能的な人工イオンチャネルの作成を実証する.

主な方法:

2つの18クラウン-6マクロサイクルの水性光駆動ロータリーモーターの機能化.
分子構造をフォスフォリピド二重層に組み込む.
イオン輸送を監視するために光測定法とパッチクランプ電生理学を使用した.

主要な成果:

フォスフォリピド二重層内の選択性アルカリイオンチャネルの形成が示された.
光照射でイオン輸送活動 (最大400%) が著しく増加した.
ロータリーモーターの均衡外動力に加速された輸送が起因する

結論:

ライト駆動の回転モーターは人工イオンチャネルを動かし,制御された膜輸送を可能にします.
分子モーターのアクチュエーションは,イオン転移のためのアクティベーションエネルギーバリアを克服します.
この研究は,光制御イオンチャネル工学の新しいアプローチを提示しています.

さらに関連する動画

09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

10.7K

11:09

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

9.7K

関連する概念動画

01:43

The Movement of Organelles and Vesicles

5.0K

In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...

5.0K

01:27

ATP Driven Pumps I: An Overview

9.0K

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...

9.0K

01:44

The Significance of Membrane Transport

34.3K

The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...

34.3K

01:37

Energy to Drive Translocation

2.3K

Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...

2.3K

01:14

Active Transport

1.3K

Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...

1.3K

01:16

Facilitated Diffusion

767

The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...

767

関連する実験動画