関連する実験動画
Updated: Oct 19, 2025

11:36
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
11.0K
ウビキチン独立のタンパク質分解による宿主発達の寄生体調節
Weijie Huang1, Allyson M MacLean1, Akiko Sugio1
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Cell
|September 18, 2021
まとめ
植物病原性フィトプラズマは SAP05 エフェクターを使用して宿主の発達を操作します. これらのタンパク質は 植物のユビキチン受容体RPN10をハイジャックし 宿主の寿命を延ばし 寄生虫の伝播を促進する異常な成長を誘発します
科学分野:
- 植物病理学
- 分子生物学
- 寄生虫学
背景:
- オブリゲート寄生虫は宿主の表型を大きく変えて伝染を強める.
- 昆虫が媒介する植物病原体によるこれらの宿主操作の背後にある分子機構は十分に理解されていません.
研究 の 目的:
- 植物プラズマからのSAP05エフェクタータンパク質が 植物の発達を制御する方法を解明する.
- SAP05で利用されるホストターゲットとメカニズムを特定します.
主な方法:
- 宿主植物におけるSAP05エフェクタータンパク質の機能を調査した.
- SAP05と宿主タンパク質の相互作用を分析した.
- タンパク質の分解経路と受容体結合を研究するために分子技術を活用した.
主要な成果:
- SAP05エフェクターは宿主の寿命を延ばし 魔女のような増殖を引き起こします
- SAP05はSPLとGATAの発達レギュレータの劣化を媒介する.
- この分解は,植物のユビキチン受容体RPN10をユビキチン化とは無関係に乗っ取ります.
- SAP05は特に植物RPN10を標的とし 昆虫ベクトルRPN10ではない.
結論:
- 単一のエフェクタータンパク質であるSAP05は宿主植物における複数の発達変化を指揮する.
- 植物プラズマは植物ユビキチン系を RPN10 経由で利用し,宿主の発達を自らの利益のために操作する.
- このメカニズムを理解することで 植物と寄生虫の相互作用や 効果因子の進化の洞察が得られます
関連する概念動画
Regulated Protein Degradation
7.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.9K
The Proteasome
1.2K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.2K
Covalently Linked Protein Regulators
8.0K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.0K
Regulation of Expression at Multiple Steps
1.1K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.1K
Receptor Downregulation in MVBs
2.3K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.3K
Regulation of Nuclear Protein Sorting
2.7K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.7K

