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Updated: Oct 17, 2025

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
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容量電子顕微鏡における全細胞器官の分割

Larissa Heinrich1, Davis Bennett1, David Ackerman1

  • 1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Nature
|October 7, 2021
PubMed
まとめ

この研究は,3D細胞再構築の自動化された方法を提示し,オルガネルの相互作用の詳細な分析を可能にします. OpenOrganelleプラットフォームは,細胞生物学の研究を進めるためのオープンアクセスデータとツールを提供しています.

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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