細胞と組織の全容電子顕微鏡のアトラス
C Shan Xu1, Song Pang2, Gleb Shtengel2
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. xuc@janelia.hhmi.org.
Nature
|October 7, 2021
まとめ
細胞構造の高解像度電子顕微鏡 (EM) アトラスを作成しました この新巻のEMアトラスは 詳細な細胞構造の研究のためのイメージング能力を大幅に拡張しています
科学分野:
- 細胞生物学
- 顕微鏡技術
背景:
- 細胞の構造を理解することは 生物学的な洞察に不可欠です
- 薄断面電子顕微鏡 (EM) やEMトモグラフィのような従来の電子顕微鏡 (EM) の方法は,高解像度で大きな細胞容量を視覚化するのに限界があります.
- 焦点イオンビームスキャニング電子顕微鏡 (FIB-SEM) は以前,4nmの同位分辨率で小さな細胞の体積をイメージした.
研究 の 目的:
- FIB-SEMを用いた4nm同位体ヴォクセルで画像化できる細胞サンプル量を大幅に増加させる.
- 細胞と組織の研究のための基礎的な,オープンな,高解像度ボリュームのEMアトラスを作成する.
主な方法:
- 先進的な焦点イオンビームスキャニング電子顕微鏡 (FIB-SEM) の技術を使用した.
- FIBの研磨の精度と安定性,信号検出の強化,およびスキャニング電子顕微鏡 (SEM) の取得の高速化.
- 細胞と組織の10個の3Dデータセットを4nmのアイソトロピック・ヴォクセル解像度で生成した.
主要な成果:
- 以前のFIB-SEM機能と比較して,4nmの同位分辨率で想像できるボリュームを2桁増加させた.
- 癌細胞,免疫細胞,臓の小島,神経組織を含む多様な生物学的サンプルを含む包括的な体積のEMアトラスを開発しました.
- OpenOrganelleプラットフォームを通じて,高解像度のデータセットを公開しました.
結論:
- 開発されたボリュームのEMアトラスは セルラーアーキテクチャに対する前例のない高解像度の洞察を提供します.
- このリソースは,将来の高解像度全細胞量EM研究と分析の基礎となる.
- アトラスを調査し,新しい生物学的発見のためにデータを活用することを研究コミュニティに奨励します.
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