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関連する概念動画

The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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Replication in Prokaryotes02:35

Replication in Prokaryotes

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ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

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Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Replication in Eukaryotes02:31

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Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
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超分子ポリメリゼーションを駆動する分子複製プロセス

Yuanning Feng1, Douglas Philp2

  • 1Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.

Journal of the American Chemical Society
|October 7, 2021
PubMed
まとめ
この要約は機械生成です。

研究者らは,自己テンプレート化,水素結合モノマーを使用して,超分子ポリマーを作成するための新しい方法を開発しました. このアプローチは合成の課題を克服し,機能的なポリマー材料の作成を可能にします.

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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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関連する実験動画

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Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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科学分野:

  • 超分子化学
  • ポリマー科学
  • 材料科学

背景:

  • 超分子ポリマーは,連鎖結合のために非共性結合を使用し,多用途を提供します.
  • 超分子ポリマーの連続した水素結合配列の合成は依然として重要な課題である.
  • 既存の制限は,超分子ポリマー技術の広範な展開を妨げています.

研究 の 目的:

  • 新しい水素結合の 超分子ポリマーを設計し合成する
  • 合成の限界を克服するために 自己テンプレートと自己触媒メカニズムを使用します.
  • 機能的な超分子材料の構築のための多岐にわたるアプローチを実証する.

主な方法:

  • 硬いスペーサーで接続された2つの複製テンプレートを持つ二機能モノマーを設計しました.
  • 高いダイアステロ選択性を持つモノマー合成のためのテンプレート指向の1,3二極サイクロアディションを使用した.
  • 拡散配列型NMRスペクトロスコーピーを用いて溶液中のポリマー組立を調査し,X線微分とスキャニング電子顕微鏡で材料を特徴付けました.

主要な成果:

  • バイ機能モノマーを 自動触媒で合成した
  • モノマーポリメリゼーションで高い結合親和性と正の協力性を示した.
  • その構造的整合性を確認する,超分子ポリマーの微晶と薄膜の形態を用意した.

結論:

  • 開発されたアプローチは,超分子ポリマーを構築するための有効な戦略を提供します.
  • セルフテンプレートメカニズムは ポリマー形成とモノマー合成を効果的に推進します
  • この方法論は,追加の機能を持つ超分子ポリマーを作成するために拡張可能です.