関連する実験動画

Updated: Oct 16, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.6K

編集結果の細胞決定因子を操作することによって,改良されたプライム編集システム

Peter J Chen1, Jeffrey A Hussmann2, Jun Yan3

  • 1Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02141, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

Cell
|October 15, 2021
PubMed
まとめ

DNAミスマッチ修復 (MMR) はプライム編集を阻害し,望ましくない副産物を引き起こします. 新しいプライム編集システム (PE4,PE5,PEmax) は一時的にMMRを阻害し,哺乳類の細胞の編集効率と精度を大幅に高めています.

キーワード:
CRISPR-Cas9について修理するゲノム編集不一致を修復するプライム編集

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