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Updated: Oct 16, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

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DNA二重鎖断裂修復の遺伝的景観をマッピングする

Jeffrey A Hussmann1, Jia Ling2, Purnima Ravisankar2

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

Cell
|October 21, 2021
PubMed
まとめ

Repair-seqを使って DNAの二重鎖断裂 (DSB) 修復経路をマッピングし ゲノム編集における新しい遺伝子関係と依存関係を明らかにしました これはゲノム整合性の理解を進め,遺伝子編集ツールを最適化します.

キーワード:
CRISPR-Cas9についてDNA 修復ダブルストランド・ブレイク機能的ゲノミクスゲノム編集

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