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関連する概念動画

General Transcription Factors01:30

General Transcription Factors

5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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関連する実験動画

Updated: Oct 15, 2025

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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NINのようなタンパク質転写因子は,豆類のノードルにおけるレヘモグロビン遺伝子を調節する.

Suyu Jiang1, Marie-Françoise Jardinaud2, Jinpeng Gao1

  • 1CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Centre for Excellence in Molecular Plant Sciences (CEMPS), Shanghai Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences, Shanghai, China.

Science (New York, N.Y.)
|October 28, 2021
PubMed
まとめ

転写因子NLP2とNINは,豆類の結節におけるレヘモグロビン発現を直接活性化します. 窒素の固定に不可欠なこのメカニズムは,保存されたプロモーターモチーフを含み,植物とヘモグロビンの相互作用で古代の起源があります.

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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
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関連する実験動画

Last Updated: Oct 15, 2025

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

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科学分野:

  • 植物 分子 生物学
  • 窒素固定
  • 交響的相互作用

背景:

  • レグヘモグロビンは,豆類の根の結節における共生的な窒素固定に不可欠である.
  • 酸素ホメオスタシスを維持し,酸素に敏感なニトロゲンゼの保護でバクテリアの呼吸の必要性をバランスをとります.

研究 の 目的:

  • 豆類のノードルにおけるレヘモグロビン遺伝子の転写調節を解明する.
  • レヘモグロビン遺伝子活性化に関与する特定の転写因子とDNAモチーフを特定する.

主な方法:

  • NINのようなタンパク質 (NLP) の転写因子 (NLP2とNIN) の役割を調査した.
  • ニートレッセンシブ要素 (NRE) に似た保存プロモーターモチーフを分析した.
  • 特定されたNREのような要素をノックアウトするためにCRISPR遺伝子編集を使用しました.

主要な成果:

  • NLP2とNINは,保存されたプロモーターモチーフを介してレヘモグロビン発現を直接活性化します.
  • このモチーフは"ダブル"のNREで,豆類の方向と位置が保たれています.
  • CRISPRによるモチーフのノックアウトにより レヘモグロビンの発現が著しく低下しました

結論:

  • NLP-レゲモグロビンモジュールは,豆芽の酸素バッファリングに不可欠です.
  • この調節モジュールは,NLPと低酸素状態で機能する非共生性ヘモグロビンとの古代の関連から生じた.