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Updated: Oct 14, 2025

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

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ナノ孔を用いた単一のアミノ酸解像度での単一のタンパク質の複数の再読み

Henry Brinkerhoff1, Albert S W Kang1, Jingqian Liu2

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2629 HZ Delft, Netherlands.

Science (New York, N.Y.)
|November 4, 2021
PubMed
まとめ

研究者らはペプチドの単一アミノ酸変化を特定するためのナノ孔ベースの新しいツールを開発しました. この単一分子ペプチドリーダーは タンパク質分析と細胞生物学の研究に高い精度を提供します.

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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