Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

5.2K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
5.2K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.6K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.6K
Nuclear Export01:42

Nuclear Export

4.0K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.0K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.7K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.9K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.9K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

6.3K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.3K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Direct visualization of native GSDMD pores reveals lipid-driven stabilization during pyroptosis.

Science advances·2026
Same author

Molecular architecture of heterochromatin at the nuclear periphery of primary human cells.

Nature communications·2026
Same author

Extrapolating molecular dynamics simulations to zero time step and across thermodynamic space.

The Journal of chemical physics·2026
Same author

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same author

Modular training resources for bioimage analysis.

Journal of microscopy·2026
Same author

pH-dependent activation of the Na<sup>+</sup>/H<sup>+</sup> antiporter NhaA and conformational dynamics of its N-terminus.

Nature communications·2026

関連する実験動画

Updated: Oct 13, 2025

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.5K

核の毛穴はセルロースで膨張し,収縮する

Christian E Zimmerli1,2,3, Matteo Allegretti1,3, Vasileios Rantos4,5

  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.

Science (New York, N.Y.)
|November 11, 2021
PubMed
まとめ

核孔複合体 (NPC) は動的にサイズを変更します. 成長する細胞では,NPCは膨張しますが,ストレス下では収縮し,核膜の緊張とNPCの形状の間の関係を明らかにします.

さらに関連する動画

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
05:47

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Published on: July 29, 2018

16.1K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.1K

関連する実験動画

Last Updated: Oct 13, 2025

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.5K
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
05:47

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Published on: July 29, 2018

16.1K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.1K

科学分野:

  • 細胞生物学
  • 構造生物学
  • バイオ物理学

背景:

  • 核孔複合体 (NPC) は,真核細胞における核細胞質輸送に不可欠である.
  • NPCは ~30の核ポリンから円筒状の構造を形成し,核と細胞質の間の分子輸送を調節する.
  • NPCのダイナミックな性質と形状の変化は完全に理解されていません.

研究 の 目的:

  • 生体細胞におけるNPCの大規模な形状の変化を調査する.
  • NPCのサイズ変化を制御する分子機構と生理学的条件を解明する.
  • 核膜の緊張とNPC構造の間のリンクを確立する.

主な方法:

  • NPCを現場で視覚化するために,冷凍電子トモグラフィーを用いた.
  • NPCの構成を再構築するために統合的構造モデリングが使用されました.
  • 指数関数的な成長,エネルギー枯渇,オスモティックストレスのような細胞条件が適用されました.

主要な成果:

  • NPCは指数関数的に成長する細胞で 膨張した形状を示した.
  • NPCは細胞のエネルギー枯渇と高圧的オスモティック・ストレスの条件下で逆転的に収縮する.
  • これらの発見は,NPCの形状が細胞の生理状態に敏感であることを示唆しています.

結論:

  • この研究は,NPCの直径が静的ではなく,細胞状態によって変化することを明らかにしています.
  • 核包膜の緊張がNPCの形状に影響するモデルが提案されています.
  • これは,核細胞プラズマ輸送のダイナミックな調節に関する新しい洞察を提供します.