RNA 自己組み立て制御 CRISPR/Cas9 機能による内生遺伝子接続の構築
Jiao Lin1, Wei-Jia Wang1, Yang Wang1
1MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Journal of the American Chemical Society
|November 17, 2021
まとめ
研究者は遺伝子発現の正確な制御のために,新しい自己組み立てRNA回路を開発しました. このシステムは 細菌や哺乳類の細胞の 細胞行動をプログラムするための 新しい遺伝子結合の 生成を可能にします
科学分野:
- 合成生物学
- 分子生物学
- 遺伝子工学
背景:
- 合成回路は 人工的な遺伝子結合を 生み出すことで 細胞の行動を設計する上で 極めて重要です
- 既存の遺伝子操作のツールボックスには,様々な内生遺伝子の汎用性と設計の容易さが欠けている.
研究 の 目的:
- 内生遺伝子制御ネットワークの直接操作のための新しい自己組み立て誘発RNA回路を提示する.
- 柔軟でシンプルなアプローチで 新しい遺伝子の結合を 示すこと
主な方法:
- CRISPR/Cas9ガイドRNAアセンブリにインスパイアされた自己組み立てRNA回路を設計した.
- CRISPR/Cas9制御のための三元ガイドRNAアセンブリを形成するために独立したトリガーRNAを使用した.
- コリ菌と哺乳類の細胞における内生性遺伝子発現調節システムを適用した.
主要な成果:
- *E. coli* の小さなRNAとmRNAを用いて,RNA回路を通じて内生性遺伝子発現を成功裏に制御した.
- 新しい遺伝子結合を導入することで 細胞のフェノタイプをプログラムすることを示した.
- 哺乳類の自己組織化RNA回路の機能を検証した.
結論:
- 自己組み立てRNA回路は,内生的な遺伝子接続を確立するための柔軟で単純な方法を提供します.
- このアプローチは 細胞の遺伝子ネットワークを 操作するための強力なツールです
- このデザインは合成生物学と遺伝子工学の応用に広範囲に及ぶ.
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