関連する実験動画
Updated: Jun 13, 2026

08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
6.9K
空間ゲノミクスは,組織におけるクローン異質性の多様式研究を可能にします
Tongtong Zhao1,2, Zachary D Chiang1,2,3, Julia W Morriss1,2
1Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Nature
|December 16, 2021
まとめ
新しいスライド-DNA-seq技術は,無傷の組織における空間的ゲノミクスをマップします. この方法は 腫瘍のクローンと 関連する遺伝子発現を明らかにし 癌の進行に関する理解を深めます
科学分野:
- ゲノミクス
- 細胞生物学
- 癌 研究
背景:
- 腫瘍の進行は遺伝的異常と 腫瘍の微小環境の影響を受けます
- これらの影響を定量化するには,空間的なゲノムおよびフェノタイプデータの正確な測定が必要です.
- 既存の技術は,組織内のゲノム変異の空間的な文脈を完全に捉えることはできません.
研究 の 目的:
- 完ぺきな組織部分で空間的に解明されたDNAの配列化のための新しい方法を開発する.
- 腫瘍のクローンと コピーナンバーの変異を 発見できるように
- 空間的ゲノミクスを空間的トランスクリプトミクスと統合し,遺伝子発現と微環境を結びつける.
主な方法:
- 無傷の組織部から空間的に解明されたDNA配列を捕捉するためのスライド-DNA-seqの開発.
- スライド-DNA-seqをマウス転移モデルとヒトの原発がんに適用する.
- スライド-DNA-seqデータと空間トランスクリプトミクスの統合.
主要な成果:
- Slide-DNA-seqは 局所的な腫瘍構造を正確に保存しています
- この方法により,異なる腫瘍のクローンとその複製数の変化を新たに発見することができました.
- クローン集団は,異なる空間領域に限定されていることが判明しました.
- 空間トランスクリプトミクスとの統合は,クローン特異的偏差,マイクロ環境,またはその両方に関連した遺伝子を特定しました.
結論:
- Slide-DNA-seqは,マルチモダルの空間ゲノミクスのための汎用性のあるプラットフォームです.
- このアプローチにより,細胞内因性および細胞外因性因子の細胞フェノタイプへの寄与を定量化することができる.
- この技術は腫瘍の異質性の空間的組織とその原動力について 新たな洞察をもたらします
関連する概念動画
Genomics
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Types of Genetic Transfer Between Organisms
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Types of Genetic Transfer Between Organisms
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...

