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Updated: Oct 8, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

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切除可能なO-リンクされたβ-N-アセチルグルコサミン戦略を用いて,正しく折りたたまれた二酸化硫化物豊富なタンパク質の化学合成

Wei-Wei Shi1, Chaowei Shi, Tong-Yue Wang1

  • 1Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, State Key Laboratory of Chemical Oncogenomics (Shenzhen), Department of Chemistry, Tsinghua University, Beijing 100084, China.

Journal of the American Chemical Society
|January 3, 2022
PubMed
まとめ

化学者は,複雑な二硫化物豊富なタンパク質の合成を簡素化するために,新しい可移性グリコシル化改変 (RGM) 戦略を開発した. この方法は折りたたみの中間物質を安定させ,ヘプシジンやインタールキン5のような治療用タンパク質の効率的な生産を可能にします.

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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins11:25

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

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Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

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