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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

21.9K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
21.9K
Protein Complex Assembly02:41

Protein Complex Assembly

12.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
12.6K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

18.6K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
18.6K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.0K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.0K

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関連する実験動画

Updated: Oct 6, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

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暗黙のネガティブデザインによる再構成可能な非対称タンパク質組

Danny D Sahtoe1,2,3, Florian Praetorius1,2, Alexis Courbet1,2,3

  • 1Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Science (New York, N.Y.)
|January 20, 2022
PubMed
まとめ

科学者は 複雑な構造に組み立てられ サブユニット交換で再構成できる 安定したタンパク質を設計しました ダイナミックで非対称なタンパク質システムを 生物学的応用のために作る 新しい方法を提示しています

さらに関連する動画

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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Origami Inspired Self-assembly of Patterned and Reconfigurable Particles
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Origami Inspired Self-assembly of Patterned and Reconfigurable Particles

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関連する実験動画

Last Updated: Oct 6, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.2K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

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Origami Inspired Self-assembly of Patterned and Reconfigurable Particles
12:33

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles

Published on: February 4, 2013

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科学分野:

  • タンパク質工学
  • 計算生物学
  • 構造生物学

背景:

  • 非対称的な多タンパク質複合体は生物学的プロセスにおいて極めて重要です.
  • このような複合体の設計は,可逆組立と孤立したコンポーネントの安定性の必要性のために困難です.

研究 の 目的:

  • 安定し,再構成可能な非対称なタンパク質複合体の設計のための一般的な戦略を開発する.
  • タンパク質の構成要素を 作り出すことで 様々な構造を 作り出すことができます

主な方法:

  • ベータシート媒介ヘテロダイマーを設計するために暗黙の負の設計を使用した.
  • リバーシブルアソシエーションのためのタンパク質インターフェースの計算モデルと設計.
  • 安定性アッセイ,アセンブリ運動学,X線結晶学を含む実験的検証.

主要な成果:

  • 安定した,折りたたまれた,溶解性のタンパク質ヘテロジマーを成功裏に設計した.
  • 設計されたコンポーネントを様々な複雑な構造 (線形,分岐,リング) に迅速に組み立てることが実証されています.
  • 結晶学で組み立てられた複合体の構造的整合性を確認し,計算モデルと密接に一致しています.
  • サブユニット交換による複合体の再構成性を示した.

結論:

  • 開発された暗黙の否定的な設計アプローチは,新しい非対称なタンパク質システムを作成するための多用途な経路を提供します.
  • 設計されたタンパク質構成要素は,ダイナミックで再構成可能な分子構造の構築を可能にします.
  • この研究は複雑な生物学的機能のための タンパク質設計の分野を前進させています