オミクロンおよびデルタ型SARS-CoV-2からRBDをスパイクするヒトACE2の受容体結合および複雑な構造

Pengcheng Han¹, Linjie Li², Sheng Liu³

¹CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Medicine, Zhongda Hospital, Southeast University, NanJing 210009, China.

Cell

|January 30, 2022

まとめ

オミクロン変種

科学分野:

ウイルス学
構造生物学
免疫学

背景:

新興のSARS-CoV-2変種によって引き起こされるCOVID-19のパンデミックは,世界的に続いている.
オミクロン (B.1.1.529) は,そのスパイクタンパク質の受容体結合領域 (RBD) に多数の変異があるため,懸念される重要な変異種 (VOC) である.

研究の目的:

人間のACE2 (hACE2) とのVOC RBDの結合特性を調査する.
オミクロンとデルタ変異のRBDとHACE2の相互作用の基礎となる構造的メカニズムを解明する.

主な方法:

結晶学と冷凍電子顕微鏡を用いて,オミクロンRBD-hACE2とデルタRBD-hACE2複合体の構造を解明した.
様々なVOC RBDとHACE2の結合親和性は定量的に評価された.

主要な成果:

オミクロンRBDは,アルファ,ベータ,ガマ変種とは異なり,原型SARS-CoV-2RBDと比べられるHACE2への結合親和性を示しています.
構造分析は,オミクロンとデルタRBDの特定の変異がHaCE2結合にどのように影響するかを明らかにしています.

結論:

Omicron RBDの結合プロファイルは,免疫回避と伝染性の間の潜在的なバランスを示唆しています.
RBD-hACE2の相互作用に関する構造的な洞察は,変種感染性を理解し,標的治療法を開発するための基盤を提供します.

キーワード:

RBD について VOC についてデルタハエCE2 オミクロン受容体結合領域構造懸念される変種

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