Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin : Application to investigations of ferredoxin inheritance in Nicotiana hybrids.

Planta·2014
Same author

Biosynthesis of ferredoxin in Chlamydomonas reinhardii.

Planta·2013
Same author

Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency.

Leukemia·2012
Same author

Risk factors and time delay associated with cardiac device infections: Leiden device registry.

Heart (British Cardiac Society)·2008
Same author

A circadian output in Drosophila mediated by neurofibromatosis-1 and Ras/MAPK.

Science (New York, N.Y.)·2001
Same author

Of flies and men--studying human disease in Drosophila.

Current opinion in genetics & development·2001

関連する実験動画

Updated: May 8, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

c-abl mRNAにおける代替5'-エクソンが存在する.

Y Ben-Neriah, A Bernards, M Paskind

    Cell
    |February 28, 1986
    PubMed
    まとめ

    タンパク質-チロシンキナーゼであるセルラーablプロトオンコゲンは,代替スプライシングにより,さまざまなmRNAサイズを示しています. このスプライシングは,フィラデルフィア染色体転位に関与する同じ部位で発生します.

    科学分野:

    • 分子生物学は分子生物学である.
    • 腫瘍生成 (オンコゲネシス) について
    • 遺伝子発現の表現について

    背景:

    • 細胞のablプロトオンコゲンは,タンパク質チロシンキナーゼをコードする.
    • この遺伝子は様々な細胞タイプで発現し,通常は2つまたは3つの異なるmRNAサイズ変異体として発現します.

    研究 の 目的:

    • マウスのc-abl cDNAの構造的多様性を調査する.
    • c-abl mRNAの異質性を生み出すメカニズムを理解する.
    • c-ablスプライシングと腫瘍性変異の関係を探求する.

    主な方法:

    • 70Z/3リンパ性細胞から4種類のマウスのc-abl補完DNA (cDNA) をクローン化しました.
    • クローンされたcDNAの5'配列を分析して,予測されたN末端アミノ酸配列を決定する.
    • c-abl cDNA配列とウイルス abl (v-abl) 配列の比較.

    主要な成果:

    • 4つの異なるマウスのc-ablcDNAが特定され,それぞれが20〜45のアミノ酸の予測されたN末端領域をコードするユニークな5'配列を有する.
    • 1つのcDNAは,v-ablのgag N端とmet-gly-gln N端の配列を共有していた.

    さらに関連する動画

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

    Published on: December 9, 2016

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    関連する実験動画

    Last Updated: May 8, 2026

    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
    11:02

    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

    Published on: May 27, 2016

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

    Published on: December 9, 2016

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

  • c-abl cDNAにおける観察された5'異質性は,3'エクソンの共通のセットに5'エクソンが加えられた代替によるものである.
  • 代替スプライシングは,フィラデルフィア染色体トランスロケーションでBCR配列がABL配列に結合する同一の場所に発生することが判明しました.

    • 結論:

    • 細胞内のablプロトオンコゲンは,mRNAレベルにおいて,著しい構造的多様性を表している.
    • 代替スプライシングは,異なる形のc-ablを生成する重要なメカニズムです.
    • c-abl異質性に関与するスプライシング部位は,フィラデルフィア染色体のような腫瘍性トランスロケーションの文脈で保存されます.