核塩基クラスタリングは,繰り返し拡張RNAコンデンサートの形成と空洞化に寄与する
Yingxue Ma1, Haozheng Li2, Zhou Gong1
1CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Center for Magnetic Resonance at Wuhan, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China.
Journal of the American Chemical Society
|February 18, 2022
まとめ
RNA分子は繰り返し膨張し ゲル状の滴を形成し 神経変性疾患を引き起こす可能性があります これらのドロップルはクラスタ化された核塩基を示し,時間が経つにつれて硬化し,RNAを含む臓器に影響を及ぼします.
科学分野:
- 生物化学
- 分子生物学
- 神経科学
背景:
- RNAの拡張シーケンスが繰り返されると,相分離とゲル状の凝縮物が形成される.
- この相分離は神経変性疾患の病原化に関連しています.
研究 の 目的:
- Mg2+の存在下で RNA 分子の自己組み立てを繰り返す拡張配列を調査する.
- RNA相分離中の形態学的および構造的変化を特徴付ける.
主な方法:
- 超スペクトル刺激ラーマン顕微鏡を用いて,RNAの滴の形成と構造を分析した.
- 変化するRNA/Mg2+比がドロップレット形態に与える影響を調査する.
主要な成果:
- 20×CAGの重複を持つRNA分子は,Mg2+の存在下で3つの異なるドロップレットタイプに自己組み立てます.
- RNA相分離は,核塩基のクラスタリングと正規の塩基配列の喪失を含みます.
- RNA/Mg2+比が増加する空洞な小胞に膨張からドロップレット形態の移行.
- 核塩基のクラスタリングはドロップレット・リムに集中し,硬化過程を示しています.
結論:
- 繰り返しの膨張と二価イオンによって引き起こされるRNA相分離により,異なる構造が形成される.
- 滴の縁で観察された硬化メカニズムは,RNAを含む臓器の老化に関連している可能性があります.
- この研究は,RNA凝縮物形成の基礎にある物理的メカニズムと,病気や老化におけるその潜在的な役割についての洞察を提供します.
関連する概念動画
02:55The Nucleolus
9.4K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
9.4K
01:25Nucleic Acid Structure
7.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
7.4K
01:23RNA Structure
74.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
74.6K
02:53Ribosomal RNA Synthesis
13.6K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.6K
03:01The Replisome
35.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
35.5K
02:43Nucleic Acids
46.5K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
46.5K