Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Peroxisomes01:24

Peroxisomes

14.8K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
14.8K
Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

3.8K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
3.8K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.2K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.2K
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

90.9K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
90.9K
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids02:04

Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

6.2K
Diols are compounds with two hydroxyl groups. In addition to syn dihydroxylation, diols can also be synthesized through the process of anti dihydroxylation. The process involves treating an alkene with a peroxycarboxylic acid to form an epoxide. Epoxides are highly strained three-membered rings with oxygen and two carbons occupying the corners of an equilateral triangle. This step is followed by ring-opening of the epoxide in the presence of an aqueous acid to give a trans diol.
6.2K
Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

8.0K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
8.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Spatially resolved profiling of steroid nuclear receptors reveals a role for the disordered N-terminal domains in genome targeting and AP-1 interaction.

Genome research·2026
Same author

The transmembrane domain structure of TNFR1 suppresses ligand-independent autoactivation but is not required for TNF-induced signaling.

Science signaling·2026
Same author

Unspecific Peroxygenases-Catalyzed Oxidation of Pharmaceutical Compounds Considered Emerging Contaminants.

Chembiochem : a European journal of chemical biology·2026
Same author

Complete biosynthesis of psychedelic tryptamines from three kingdoms in plants.

Science advances·2026
Same author

Optimizing Stability in Dynamic Small-Molecule Binding Proteins.

Journal of the American Chemical Society·2025
Same author

Engineering the Tobacco Etch Virus Protease toward a Platform for Traceless Cleavage Using Distal Site Prediction and Smart Library Design.

ACS synthetic biology·2025
Same journal

Proton-Gated Torsional Spring for Molecular Energy Storage.

Journal of the American Chemical Society·2026
Same journal

Topologically Programmed Dual-Channel Covalent Organic Frameworks Decouple Gas and Ion Fluxes for Acidic CO<sub>2</sub> Electroreduction.

Journal of the American Chemical Society·2026
Same journal

Plasmonic Re-Excitation Enables Superoxide-Mediated Ethane Conversion to Acetic Acid under Visible Light.

Journal of the American Chemical Society·2026
Same journal

Photocatalytic Controlled Halodefluorination of Perfluoroalkyl Compounds Using <i>N</i>-Arylphenothiazines.

Journal of the American Chemical Society·2026
Same journal

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same journal

Biocatalytic C3 β-<i>O</i>-Glycosylation of Triterpenes and Sterols to Synthesize Natural and Unnatural Saponins.

Journal of the American Chemical Society·2026
関連記事をすべて見る

関連する実験動画

Updated: Oct 3, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

8.6K

シーケンスから直接設計された安定で機能的に多様な多用途ペロキシダース

Shiran Barber-Zucker1, Vladimir Mindel1, Eva Garcia-Ruiz2

  • 1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel.

Journal of the American Chemical Society
|February 18, 2022
PubMed
まとめ
この要約は機械生成です。

AIで予測された構造を用いた計算型酵素設計により,難易度の高い多機能過酸化酵素 (VP) の機能的発現が可能になった. この進歩により,工業的な応用のための酵素ファミリーのより広範な探求が可能になります.

さらに関連する動画

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.5K
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K

関連する実験動画

Last Updated: Oct 3, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

8.6K
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.5K
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K

科学分野:

  • 生物化学
  • 酵素工学
  • コンピュータ生物学

背景:

  • 白腐菌は酸化還元酵素,特に多用性過酸化酵素 (VPs) を利用し,効率的なリグニン分解を行います.
  • VPの再結合生産は困難であり,研究と産業の応用を制限しています.
  • 精密な酵素構造は計算上の最適化に不可欠ですが,多くの酵素には実験的な構造が不足しています.

研究 の 目的:

  • 計算型酵素設計のためのディープラーニングベースの*ab initio*構造予測の信頼性を評価する.
  • 改良された性質を持つ新しい多用途ペロキシダゼ (VPs) を設計し,機能的に発現させる.
  • 自然の酵素の多様性を探求するためのAI駆動の方法の有用性を実証する.

主な方法:

  • VP最適化のためのモデルを生成するために,ディープラーニング *ab initio* 構造予測を使用しました.
  • VPのワンショット安定性と機能強化のためのPROSS計算設計を採用しました.
  • 酵母における設計されたVP変種を表現し,その活性,安定性,反応性プロフィールを特徴づけた.

主要な成果:

  • AIが予測した構造は コンピューティングによる酵素設計の 信頼できる出発点として機能しました
  • 最多43の変異を持つ4つの設計されたVPは,ワイルドタイプの同類のものとは異なり,酵母で成功裏に発現しました.
  • 3つの設計は,反応性と環境耐性において顕著な多様性を示した.

結論:

  • ディープラーニングの構造予測と計算設計は 複雑な発現を持つ酵素の効率的な最適化を可能にします
  • このアプローチは,計算による酵素工学の範囲を拡大し,新しい生物触媒の発見を容易にする.
  • この方法論は,ゲノムデータから天然の酵素ファミリー内の機能的多様性を直接利用することを可能にします.