関連する実験動画

Updated: Aug 14, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

酵母からの単一の遺伝子は,核および細胞質のポリアデニラート結合タンパク質の両方のために:ドメイン構造と発現

A B Sachs, M W Bond, R D Kornberg

    Cell
    |June 20, 1986
    PubMed
    まとめ

    研究者らは,Saccharomyces cerevisiae poly(A) 結合タンパク質の遺伝子を特定し,ユニークなトランスクリプトを生成する単一コピー遺伝子を明らかにしました. 核タンパク質は,細胞プラズマの前駆体分裂の結果であり,DNA配列は保全されたポリ (A) 結合ドメインと潜在的翻訳フィードバック調節を示す.

    さらに関連する動画

    A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions14:23

    A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

    Published on: June 6, 2018

    Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers10:28

    Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

    Published on: December 6, 2019

    関連する概念動画

    Yeast Signaling01:28

    Yeast Signaling

    Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
    Conservation of Protein Domains Over Different Proteins02:26

    Conservation of Protein Domains Over Different Proteins

    Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
    A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
    Protein Complexes with Interchangeable Parts01:57

    Protein Complexes with Interchangeable Parts

    Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
    The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
    Regulated mRNA Transport02:22

    Regulated mRNA Transport

    In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
    Regulation of Nuclear Protein Sorting01:45

    Regulation of Nuclear Protein Sorting

    Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...