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S. flexneri 2aの大きなプラズミド上の隣接する細胞の継続的な再感染に必要な遺伝的決定因子
Cell
|August 15, 1986
まとめ
Shigella flexneriの毒性プラズミドの新たに特定された領域,virGは,細菌の細胞から細胞への拡散に不可欠である. virGの変異は,隣接する細胞の感染を防止し,細菌の絶滅につながります.
科学分野:
- 微生物学 微生物学とは
- バクテリア病原菌の発生
- 分子生物学は分子生物学である.
背景:
- Shigella flexneriは,バシラ性痢疾を引き起こす病原体である.
- 細菌の毒性の要因は,宿主細胞の侵入と拡散に極めて重要です.
- バクテリアの拡散の遺伝的基盤を理解することは,介入の開発の鍵です.
研究 の 目的:
- Shigella flexneri.の細胞間感染の遺伝的決定因子を特定する.
- 特定されたウイルス性遺伝子領域の機能を特徴付けるために.
主な方法:
- トランポゾン突然変異 (Tn5) は,細胞間拡散の欠陥を持つ変異体を特定するために使用されました.
- ホストの上皮細胞内の細菌変異体の局所化と特徴化.
- 特定された領域の他の細菌株における有病率を決定するハイブリッド化研究.
主要な成果:
- S. flexneriの毒性プラズミドの特定の領域, virGを指定した領域は,細胞から細胞への感染に不可欠であると特定されました.
- virGへのTn5挿入は,上皮細胞に侵入し,増殖したが,拡散することはできなかったアビルラント変異を生じさせた.
- これらの変異体は運動性を失い,細胞内局所化し,隣接する細胞に感染することなく消滅した.
- 約4kbのvirG領域は,単一の遺伝子を含んでおり,他のシゲッラ菌および腸内侵入性E. coliウイルス性プラズミドに保存されています.
結論:
- virG領域は,Shigella flexneriの細胞間感染を決定する重要な決定因子である.
- virGは,細菌の運動性や細胞間侵入に不可欠な因子をコードしている可能性が高い.
- virGの保存は,Shigellaおよび関連する細菌の病原性におけるその重要性を示唆しています.
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