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関連する概念動画

Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

5.1K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
5.1K
Facilitated Diffusion01:16

Facilitated Diffusion

683
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
683
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

575
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
575
Membrane Domains01:18

Membrane Domains

6.0K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.0K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.1K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.1K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.4K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.4K

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関連する実験動画

Updated: Sep 29, 2025

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

9.8K

ブロードバンド膜キャリアとしてのボロンクラスター

Andrea Barba-Bon1, Giulia Salluce2, Irene Lostalé-Seijo2

  • 1Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

Nature
|March 24, 2022
PubMed
まとめ

B12Br12(2-のような球状ドデカボレートクラスターは,水性分子のための新しい無機媒介体として作用する. これらの超カオトロプアニオンは 膜を横断して輸送し,薬の投与と細胞のラベル付けを可能にします.

さらに関連する動画

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

9.8K

関連する実験動画

Last Updated: Sep 29, 2025

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

9.8K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.1K
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

9.8K

科学分野:

  • 生物化学
  • 材料科学
  • ナノテクノロジー

背景:

  • 水性物質の膜転移は,治療薬や探査機にとって難しい.
  • 従来のアンフィフィリックキャリアは,蓄積と膜解離を引き起こす可能性があります.
  • これらの制約を克服するために新しいキャリアが必要です.

研究 の 目的:

  • 球状ドデカボラートクラスターを無機膜媒介体として調査する.
  • 水性貨物の輸送能力を評価する.
  • 細胞伝達におけるこれらのキャリアの潜在的応用を探求する.

主な方法:

  • 膜輸送を評価するためにリポソームモデルを使用した.
  • ペプチド,アミノ酸,神経伝達物質,ビタミン,抗生物質,医薬品を含む特有の貨物タイプ.
  • 運搬活動を理解するために 機械的な運搬研究を行いました
  • 生体細胞における生物活性分子の細胞吸収が実証されている.

主要な成果:

  • ドデカボラートクラスター,特にB12Br12-は,アニオンの無機膜媒介体として機能する.
  • リポソーム膜に広範囲の水性分子 (146-4,500Da) を成功裏に輸送した.
  • キャリア活動はクラスターアニオンの超カオトロプ性と相関する.
  • 細胞内における細胞骨格の標識化のためのプロテオリシス標的キメラとファロイドイン毒素の投与を示した.

結論:

  • 球状のドデカボレートクラスターは,新しい種類の無機膜媒体を提供します.
  • 超カオトロプ的特性により,広域の水性輸送が可能である.
  • これらのキャリアは細胞生物学,神経生物学,生理学,医薬品の潜在的応用があります.