酵母カルモジュリン遺伝子の分離:カルモジュリンは不可欠なタンパク質です

T N Davis, M S Urdea, F R Masiarz

Cell

|November 7, 1986

まとめ

細胞成長に不可欠な酵母カルモジュリンを浄化し,その遺伝子を分離しました. このタンパク質は,他のカルモジュリンと60%の同一性を共有し,その遺伝子の破壊は致命的な変異を引き起こす.

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
イースト遺伝学イースト遺伝学

背景:

カルモジュリンは,細胞信号伝達に関与する重要なカルシウム結合タンパク質です.
Saccharomyces cerevisiaeにおけるカルモジュリンの役割を理解することは,真核細胞の機能についての洞察を提供します.

研究の目的:

Saccharomyces cerevisiaeからカルモジュリンを精製し,特徴づけました.
酵母カルモジュリンをコードする遺伝子を分離し,分析する.
酵母細胞の生存能力に対するカルモジュリンの必要性を決定する.

主な方法:

カルモジュリンの浄化は,Ca2+に依存する性質に基づいています.
合成オリゴヌクレオチドプローブを用いたカルモジュリン遺伝子 (CMD1) の分離.
致死性を評価するための遺伝子破壊と消去.

主要な成果:

酵母カルモジュリンは,Ca2+結合と電泳性移動性シフトを含む,他のカルモジュリンと類似した性質を示しています.
CMD1遺伝子は,II染色体上の単一複製の位置であり,イントロンが欠けています.
イーストカルモジュリンは,他のカルモジュリンと60%のアミノ酸同一性を共有しています.
CMD1遺伝子の破壊は,後退性致死性突然変異を引き起こす.

結論:

カルモジュリンは,Saccharomyces cerevisiaeの成長と生存に不可欠です.
孤立したCMD1遺伝子は,酵母におけるカルモジュリン機能のさらなる研究のためのツールを提供します.
酵母カルモジュリンは進化的に保存され,他のカルモジュリンと有意な配列ホモロジーを持っています.

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