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RNA Stability01:53

RNA Stability

34.0K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.0K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.8K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

10.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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RNA Editing02:23

RNA Editing

9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
pre-mRNA Processing02:01

pre-mRNA Processing

53.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
53.8K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.9K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.9K

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Updated: Sep 28, 2025

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
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Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

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N6-メチラデノシンがVSGのトランスクリプトを安定させる

Idálio J Viegas1, Juan Pereira de Macedo1, Lúcia Serra1

  • 1Instituto de Medicina Molecular-João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.

Nature
|March 31, 2022
PubMed
まとめ

N6-メチラデノシン (m6A) RNAの改変は,Trypanosoma bruceiの遺伝子発現を調節し,特に多様性の表面グリコプロテインのトランスクリプトのポリ (A) 尾内にあり,mRNAの安定性に影響を及ぼします.

さらに関連する動画

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

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関連する実験動画

Last Updated: Sep 28, 2025

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
08:16

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K

科学分野:

  • 分子生物学
  • 寄生虫学
  • RNA 生物学

背景:

  • トリパノソーマ・ブルセイの遺伝子発現は,ポリシストロニックな転写により,主に転写後の調節されます.
  • N6-メチラデノシン (m6A) はTrypanosoma bruceiで特定されていますが,その機能的な役割は理解されていません.

研究 の 目的:

  • トリパノソーマ・ブルセイにおける m6A RNA 変異の機能を調査する.
  • m6A改変に関連したトランスクリプトと規制要素を特定する.

主な方法:

  • m6Aを濃縮したトランスクリプトを識別するためのRNA免疫降水.
  • m6Aに関連したシーケンスモチーフを特定するための計算分析.
  • 特定されたモチーフの役割を評価するための遺伝子操作

主要な成果:

  • m6A変異は,変異表面グリコプロテイン (VSG) をコードするトランスクリプトを含む342で強化されています.
  • m6Aの約50%は,分解前に除去されたVSGトランスクリプトのポリ (A) 尾に含まれています.
  • VSG遺伝子の3'未翻訳領域の16-メルモチブは,m6Aのポリー (A) 尾への含有に不可欠である.

結論:

  • この研究では,eukaryotic mRNAのポリー (A) テイル内のm6A変異を特定し,これは転写後の遺伝子調節のための新しいメカニズムである.
  • 16 メルモチブは,m6Aの組み込みおよびその後のmRNAの安定性を制御するシス作用の要素として作用する.