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関連する概念動画

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
506
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

179
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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DNA Bacteriophages01:26

DNA Bacteriophages

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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関連する実験動画

Updated: Sep 28, 2025

Folding and Characterization of a Bio-responsive Robot from DNA Origami
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Folding and Characterization of a Bio-responsive Robot from DNA Origami

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共同対称CRISPR/dCasシステムによって折りたたまれた遺伝的にコードされた二重鎖DNAベースのナノ構造

Tiantian Wu1,2, Yuanwei Cao3,4, Qing Liu1

  • 1CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

Journal of the American Chemical Society
|March 31, 2022
PubMed
まとめ

研究者らは,クラスタリングされた定期間隔の短いパリンドロミック繰り返し (CRISPR) と二重鎖DNAを用いた新しいDNAナノテクノロジー戦略を開発した. この方法は,潜在的な遺伝子調節アプリケーションのための新しいハイブリッドナノ構造を作成します.

さらに関連する動画

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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関連する実験動画

Last Updated: Sep 28, 2025

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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科学分野:

  • バイオテクノロジー
  • ナノテクノロジー
  • 分子生物学

背景:

  • DNAナノテクノロジーは通常,ナノ構造の構築のために単鎖DNAハイブリッド化を使用します.
  • 既存の方法は複雑で安定したDNAナノ構造を作るのに 限界があります

研究 の 目的:

  • 二重鎖のDNA-リボ核タンパク質 (RNP) ハイブリッドナノ構造を構築するための新しい戦略を導入する.
  • DNAの折りたたみのための共価双価クラスタ化された定期的な間隔の短いパリンドロミックリピート (CRISPR) /ヌクレアースデッドCRISPR関連タンパク質 (dCas) システムを利用する.

主な方法:

  • dCas9とdCas12aの融合により,刺激反応性ペプチドリンクナーにより,二価RNPが生成される.
  • 誘導RNAによってRNPステップルの活性化により,二重鎖のDNA基板に特定の配列を標的にし,結合させる.
  • RNPの認識と結合を通じてDNAの折り畳みを誘導し,ハイブリッドナノ構造を形成する.

主要な成果:

  • DNA-RNPハイブリッドナノ構造の構築に成功しました
  • 折りたたまれた状態で遺伝情報を保護するナノ構造の能力の実証
  • ナノ構造の展開時に刺激に反応する遺伝子転写を示す.

結論:

  • 開発された戦略は,CRISPRベースのRNPシステムで二重鎖DNA折り畳みを採用することにより,DNAナノテクノロジーの新しいアプローチを提供します.
  • この方法は,安定したナノ構造の形成と制御された遺伝子調節のための遺伝的にコードされたプラットフォームを提供します.
  • この発見は,高度な機能的なDNAナノデバイスを設計するための新しい道を開きます.