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関連する概念動画

Next-generation Sequencing03:00

Next-generation Sequencing

93.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
93.2K
Genomics02:02

Genomics

37.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
37.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.6K
Sanger Sequencing01:57

Sanger Sequencing

759.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
759.5K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.3K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.3K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

19.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Updated: Sep 28, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

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次世代のヒトゲノム配列

Deanna M Church1

  • 1Inscripta, Inc., Boulder, CO, USA.

Science (New York, N.Y.)
|March 31, 2022
PubMed
まとめ
この要約は機械生成です。

ほぼ完全なヒトゲノム配列は 新たな基準となり より包括的な遺伝子研究と理解の道を開きます この進歩は,ゲノム研究における代表性を改善することを目的としています.

さらに関連する動画

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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関連する実験動画

Last Updated: Sep 28, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

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科学分野:

  • ゲノミクス
  • バイオ情報学
  • 人口遺伝学

背景:

  • ヒトゲノム参照配列は 遺伝学研究において極めて重要です
  • 現在のリファレンスゲノムには多様性がないため 研究結果に偏りがあります
  • より包括的で代表的なヒトゲノム参照が必要である.

研究 の 目的:

  • ほぼ完全なヒトゲノム配列を 提示するために
  • より包括的なゲノム参照の基盤を確立する.
  • ヒト遺伝学の研究におけるより広範な代表性を促進する.

主な方法:

  • 先進的なシーケンシング技術が採用されました.
  • 配列の組み立てと分析のためにバイオ情報パイプラインが利用されました.
  • 比較ゲノミクスのアプローチは,包摂性を評価するために使用されました.

主要な成果:

  • 高品質でほぼ完全なゲノム配列が生成されました.
  • 新しい配列は,以前は参照ゲノムから欠けている領域を組み込みます.
  • 初期分析では,多様な集団の代表が改善されたことが示唆されています.

結論:

  • 開発されたゲノム配列は,より包括的な人間基準への重要な一歩を表しています.
  • このリソースは 遺伝子研究におけるバイアスを 減らす可能性を秘めています
  • 世界的に代表的なゲノム参照を完全に実現するには,さらなる努力が必要です.