関連する実験動画
Updated: Sep 21, 2025
19:56
Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
15.3K
フォト反応性トランスメブランアニオントランスポーターリレー
Toby G Johnson1, Amir Sadeghi-Kelishadi1, Matthew J Langton1
1Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, U.K.
Journal of the American Chemical Society
|June 2, 2022
まとめ
研究者らは,脂質膜を横断する光制御イオン輸送のための合成分子機械リレーを開発した. この光反応システムは 望遠鏡の腕を使って 生物学的ポンプを模倣して アニオン通過を逆向きに制御します
科学分野:
- 超分子化学
- 膜生物物理学
- 分子機械
背景:
- 生物学的イオン輸送は ATPasesのようなチャネルと分子機械に依存しています
- 合成膜を横断するイオン流を制御することは,バイオミメティックアプリケーションにとって極めて重要です.
- 刺激に反応するシステムは 分子プロセスの正確な制御を提供します
研究 の 目的:
- 合成分子機械のようなイオン輸送リレーを設計し実証する.
- リピッド二重層を横切って 光を制御した逆転性イオン輸送を実現する.
- 協力的な分子機械の支架として脂質膜の使用を調査する.
主な方法:
- 脂質二層の反対側にあるトランスポーターで 分子リレーを作りました
- フォトレスポンシブな望遠鏡の腕を リレーシステムに組み込みました
- 腕の伸縮と収縮を制御するために 青と緑の光の照射を使用しました
主要な成果:
- 合成リレーは 脂質膜にアニオン輸送を成功させました
- 移動は光によって逆向きに制御され,望遠鏡の腕を伸ばしたときにのみ発生しました.
- 腕の収縮状態は 効果的なイオン輸送を阻害し 刺激反応を示した.
結論:
- 制御されたイオン輸送のための新しい刺激反応分子アンサンブルを開発した.
- 分子機械の組織化と制御のためのプラットフォームとして脂質二層の可能性を実証した.
- この研究は,バイオミメティックイオン輸送と分子デバイスのアプリケーションに新しい道を開きます.
さらに関連する動画
08:55Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
Published on: February 17, 2023
3.3K
09:35Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
Published on: November 29, 2014
22.9K
関連する概念動画
01:29Primary Active Transport
11.1K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
11.1K
01:19Membrane Asymmetry Regulating Transporters
5.0K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
5.0K
01:31Membrane Transporters
13.2K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
13.2K
01:55Secondary Active Transport
126.3K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
126.3K
01:31ABC Transporters: Exporter
5.0K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
5.0K
01:14Active Transport
927
Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
927