非コーディング7SRNAは,ミトコンドリアRNAポリメラーゼ二分化による転写を阻害する
Xuefeng Zhu1, Xie Xie1, Hrishikesh Das2
1Department of Medical Biochemistry and Cell Biology, University of Gothenburg, PO Box 440, 405 30 Gothenburg, Sweden.
Cell
|June 6, 2022
まとめ
7S RNAは,非コーディングミトコンドリア分子であり,POLRMT二分化を誘導することによってミトコンドリア転写を調節する. この相互作用は,転写の開始を妨げ,哺乳類の細胞に負のフィードバックループを形成する.
科学分野:
- ミトコンドリア生物学
- 分子遺伝学
- RNA生物学
背景:
- ミトコンドリアゲノムの13の酸化リン酸化成分は 細胞のエネルギー生産に不可欠です
- 制御不能なミトコンドリアの転写は多くのヒトの病気と関連している.
- 7S RNAは,非コーディングRNAであり,生理学的変化によって急速に調節されます.
研究 の 目的:
- ミトコンドリアの転写における7S RNAの調節作用を調査する.
- 7S RNAがミトコンドリアの遺伝子発現を制御するメカニズムを解明する.
主な方法:
- インビトロ転写測定法
- 培養細胞実験で
- 構造的な相互作用を決定するために,冷凍電子顕微鏡 (冷凍-EM) を使用する.
主要な成果:
- 7S RNAはミトコンドリアの転写レベルを in vitro と培養細胞の両方で調節することが判明しました.
- Cryo-EMは,7S RNAがRNAポリメラーゼ (POLRMT) の二分化を誘導することを明らかにした.
- 7S RNAの結合により形成されるPOLRMT二元インターフェースは,プロモーターの認識と解き放たれを阻害し,転写を阻害する.
結論:
- 7S RNAは,哺乳類の細胞におけるミトコンドリア転写の負の調節剤として作用する.
- 7S RNA-POLRMTの相互作用は,ミトコンドリアの遺伝子発現制御のためのネガティブなフィードバックループの重要な構成要素を形成する.
さらに関連する動画
関連する概念動画
Types of RNA
6.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
6.6K
siRNA - Small Interfering RNAs
17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
RNA Interference
26.5K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.5K
Riboswitches
8.7K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.7K
Transcriptional Regulation: Riboswitches
128
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
128
MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K


