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Updated: Sep 20, 2025

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

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シングル・ターンオーバー酵母チアミンピリミジン合成のメカニズム研究:不活性酵素の特徴

Rung-Yi Lai1, Anushree Mondal1, Dmytro Fedoseyenko1

  • 1Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.

Journal of the American Chemical Society
|June 8, 2022
PubMed
まとめ

ユカリオットチアミンピリミジン合成酵素 (THI5p) は,その活性部位ヒスティジンとピリドクサールリン酸 (PLP) をチアミンピリミジン (HMP-P) に変換する. 研究者は 重要な断片と 鉄の共因を特定し 反応機構を提案しました

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Turnover Number and Catalytic Efficiency

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