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Updated: Sep 8, 2025

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
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Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Published on: November 16, 2016

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ペプチドグリカンの成熟は外膜タンパク質の組成を制御する

Gideon Mamou1, Federico Corona2,3, Ruth Cohen-Khait1

  • 1Department of Biochemistry, South Parks Road, University of Oxford, Oxford, UK.

Nature
|June 15, 2022
PubMed
まとめ

グラム陰性細菌のペプチドグリカン構造は外膜タンパク質 (OMP) の挿入を誘導する. 成熟したペプチドグリカンは,OMPの挿入を制限し,細胞の成長中にOMPが優先的に挿入されるようにします.

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Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

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Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
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Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Protein Transport to the Outer Chloroplast Membrane

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