関連する実験動画

Updated: Sep 3, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

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凍り込み電子トモグラフィー: 細胞の内部空間への長い旅

Wolfgang Baumeister1

  • 1Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Cell
|July 22, 2022
PubMed
まとめ

クリオ電子トモグラフィー (cryo-ET) は,細胞の高解像度3Dイメージングを提供します. このテクニックは 細胞構造と分子構造を ほぼ本来の状態で明らかにします

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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