ペプチド-RNAコアセルバートは,折りたたまれたドメインの進化のカレッジである

Manas Seal¹, Orit Weil-Ktorza², Dragana Despotović³

¹Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 7610001, Israel.

Journal of the American Chemical Society

|July 29, 2022

まとめ

ペプチド-RNAコアセルバートはペプチド二分化とアルファヘリル折り合いを促進し,これらの初期のコンパートメントは重複と融合を通じてタンパク質の進化を助長したことを示唆しています.

科学分野:

生物化学
生命の起源に関する研究
構造生物学

背景:

ペプチド-RNAコアセルバートは濃縮されたバイオポリマーであり,早期のタンパク質進化の場として機能する可能性がある.
ペプチド構成の探査に対するコアセルバートの影響はほとんど不明である.

研究の目的:

ペプチド-RNA共生体内のヘリックス-ヘリックス (HhH) モチーフに対するRNAの構造的および動的効果を調査する.
ペプチドオリゴメリゼーションとタンパク質構造の進化におけるコアセルバートの役割を調査する.

主な方法:

電子パラマグネティック共振 (EPR) スペクトル,特にダブル電子-電子共振 (DEER) が使用されました.
シングルおよびダブルスピンラベルのHhHペプチドは単独およびペプチド-RNAコアセルバットの中で研究されました.

主要な成果:

HhHモチーフは,RNAの結合により強化された二元化により,RNAなしでも二元化を形成する.
ペプチドRNAコアセルバット内でディマー形成と一時的なアルファヘリル構造が観察されました.
結果は複製と融合によって形成された (HhH) 2折れと一致する.

結論:

ペプチド-RNAコアセルバートはペプチドのオリゴメリゼーションと構造変化を促進する.
これらのコアセルバートは複製と融合による複雑なタンパク質構造の進化に不可欠な環境を提供したのかもしれない.

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