アラビドプシスのメオティック脱出は,P体媒介による翻訳阻害によって引き起こされる

Albert Cairo¹, Anna Vargova¹, Neha Shukla¹

¹Central European Institute of Technology (CEITEC), Masaryk University, 625 00 Brno, Czech Republic.

Science (New York, N.Y.)

|August 4, 2022

まとめ

アラビドプシスの微生物分裂は翻訳阻害によってミトーシスに移行する. タンパク質TDM1はP体における翻訳開始因子を隔離し,メオシスの後の細胞運命を制御する.

科学分野:

植物生物学
細胞・分子生物学
遺伝学

背景:

細胞分裂機構の再プログラムを必要とする二倍体と二倍体間のメオシス移行.
ミト分裂後の発達には,ミト細胞分裂サイクルへの戻りが含まれます.

研究の目的:

アラビドプシスのメイオシスからミトシスへの移行を調節する分子メカニズムを調査する.
メイオティック分裂を終了し,メイオティック後の発達を開始する重要なタンパク質と経路を特定する.

主な方法:

アラビドプシスにおける三次元変異1 (TREE-DIVISION MUTANT 1) のメオシス特異性タンパク質の役割を調査した.
変換制御を研究するための焦点としてP体 (処理体) を利用した.
タンパク質の相互作用,特にTDM1とSUPRESSOR WITH MORPHOGENETIC EFFECTS ON GENITALIA 7 (SMG7) を調べました.
翻訳開始複合体eIF4Fと全体的な翻訳率に対するTDM1の影響を評価した.
tdm1変異体におけるメオティック終結に対する化学翻訳阻害の効果を分析した.

主要な成果:

アラビドプシスのメオシスからミトシスへの移行の重要なレギュラーとしてTDM1を特定した.
TDM1はSMG7との相互作用によってP体に組み込まれていることが示された.
TDM1はP体内の翻訳開始複合体eIF4Fを隔離し,翻訳を阻害することを示した.
化学的に翻訳を阻害することで TDM1変異体における介質終結の欠陥を修復することが確認されました

結論:

TDM1を含むP体は,メオティックトランスクリプトの発現をダウン調節する.
このトランスレーション制御メカニズムは,メオシスからメオシス後のゲメトファイトの分化への細胞運命の移行を容易にする.
この研究は,植物繁殖中の制御されたトランスレーションを通じて細胞サイクル移行を制御する新しいメカニズムを明らかにしています.

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