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関連する概念動画

ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.5K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.5K
ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

5.1K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
5.1K
Primary Active Transport01:29

Primary Active Transport

10.8K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
10.8K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

3.9K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.9K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.8K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.8K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.1K

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関連する実験動画

Updated: Sep 1, 2025

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
13:40

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

12.0K

トランスミダーション駆動分子ポンプ

Lorna Binks1, Chong Tian1, Stephen D P Fielden1

  • 1Department of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.

Journal of the American Chemical Society
|August 18, 2022
PubMed
まとめ
この要約は機械生成です。

研究者らは,段階的な情報ラッチメカニズムを使用して,新しい合成分子ポンプを開発しました. これらのポンプはマクロサイクルの基質を効率的に分離し,中間の脱線なしで複雑なロタキサンを制御された順次アセンブリを可能にします.

さらに関連する動画

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
10:43

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

22.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

関連する実験動画

Last Updated: Sep 1, 2025

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
13:40

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

12.0K
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
10:43

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

22.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

科学分野:

  • 超分子化学
  • 合成有機化学
  • ナノテクノロジー

背景:

  • 分子ポンプは 制御された分子操作に不可欠です
  • 以前の方法はしばしば非効率な封じ込めや連続組み立てに対する制御の欠如に苦しんでいます.
  • マクロサイクル基板には,制御された結合と放出のための特定のメカニズムが必要です.

研究 の 目的:

  • 新種の合成分子ポンプを報告する
  • 動力ゲートのための段階的な情報ラッチメカニズムを実証する.
  • マクロサイクリック基質の効率的な封じ込めと制御されたロタキサン合成を達成するために.

主な方法:

  • ポンプ端のアミンとアシル電極の間の活性テンプレート反応を利用した.
  • カーボキシル化とカーバマートからフェノールエステルへの変換が,連続的なポンプサイクルに使用されている.
  • 単結晶X線微分法を用いた合成ロタキサンの構造的特徴化.

主要な成果:

  • マクロサイクルを1つまたは両端からスレッドにラッチングできる分子ポンプを開発した.
  • ポンプサイクルの末端アシルグループごとに1つの追加リングが達成された.
  • 3つの異なるマクロサイクルの [4] ロタキサンと [5] ロタキサンを合成し,X線微分によって安定作用を示すことが確認された.

結論:

  • 新しい分子ポンプは,段階的な情報ラッチメカニズムを効果的に利用します.
  • シドオロタキサン状態の欠如は,マクロサイクルの効率的で制御された連続的な負荷を確保します.
  • この方法論は,複雑で配列で定義されたロタキサンの正確な合成を容易にする.