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関連する概念動画

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Galvanometer01:24

Galvanometer

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Common devices, including car instrument panels, battery chargers, and inexpensive electrical instruments, measure potential difference (voltage), current, or resistance using a d'Arsonval galvanometer. This electromechanical instrument is also known as a moving coil galvanometer.
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Updated: May 6, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
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2フォトン顕微鏡のために進化した高速インジケーターを使用して,持続的な深層組織電圧記録

Zhuohe Liu1, Xiaoyu Lu2, Vincent Villette3

  • 1Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USA.

Cell
|August 19, 2022
PubMed
まとめ
この要約は機械生成です。

2フォトン顕微鏡の性能を大幅に改善し 更に深く精密な神経活動監視を可能にしました

キーワード:
GEVI についてJEDI-2Pについてフライビジョン遺伝的にコードされた電圧指示器高通量スクリーニング双方向の電圧相関ランダム アクセス 顕微鏡スターバーストアマクリン細胞2フォトン光顕微鏡電圧イメージング

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関連する実験動画

Last Updated: May 6, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

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In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin
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科学分野:

  • 神経科学
  • バイオテクノロジー
  • 顕微鏡検査

背景:

  • 遺伝的にコードされた電圧指標 (GEVIs) は,セル型特異的な電圧ダイナミクスの監視に不可欠です.
  • 現在のGEVIは,特に2フォトン顕微鏡で,深層組織の神経記録を阻害する性能の限界を示しています.

研究 の 目的:

  • 2フォトン顕微鏡で性能を向上させるためにGEVIを最適化します.
  • 速度,明るさ,感度,光安定性を改善した新しいGEVI,JEDI-2Pを開発する.

主な方法:

  • GEVIの最適化のための多パラメータ高通量プラットフォームの開発.
  • JEDI-2Pの識別と特徴付けのためのプラットフォームの適用.
  • ドロソフィラ,マウスの網膜,そして目覚めたマウスのJEDI-2Pのin vivoおよびex vivo検証.

主要な成果:

  • JEDI-2Pは,既存の指標と比較して優れた速度,明るさ,感度,および光安定性を示しています.
  • ドロソフィラ内ニューロンとマウスの網膜のアマクリン細胞で,光誘発反応が報告されました.
  • 覚醒したマウスの個々の皮質ニューロンの長期の光学記録を,共振スキャンとULoVE顕微鏡を用いて可能にしました.
  • JEDI-2PによるULoVE記録は,深さ400μm以上のピークを検出し,神経電圧の相関を明らかにした.

結論:

  • JEDI-2Pは,2光子顕微鏡のためのGEVIの重要な進歩を表しています.
  • このインジケータは,深部神経回路における高解像度の電圧イメージングを容易にする.
  • JEDI-2Pは,神経回路の機能を研究するための新しい道を開きます.