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関連する概念動画

Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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ホストとゲストの相互作用によって引き起こされるDNA鎖の移動

Dilanka V D Walpita Kankanamalage1, Jennifer H T Tran2, Noah Beltrami1

  • 1Department of Chemistry, Tulane University, 2015 Percival Stern Hall, New Orleans, Louisiana 70118, United States.

Journal of the American Chemical Society
|September 5, 2022
PubMed
まとめ
この要約は機械生成です。

この研究では,キュキュルビト[7]ウリルを用いた新しいDNA化学方法である宿主-ゲスト駆動型トーホールド媒介性鎖移位 (HG-TMSD) を導入した. HG-TMSDは,高度なDNAマシンとバイオセンサのための調整可能な制御を提供します.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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科学分野:

  • 合成生物学
  • 超分子化学
  • ナノテクノロジー

背景:

  • 塩基対駆動型トホールド媒介性鎖移位 (BP-TMSD) は,DNAマシンとネットワークにとって極めて重要です.
  • 既存の方法は,ダイナミックなDNA化学の応用に限界があります.

研究 の 目的:

  • ホスト・ゲスト・ドリブル・トゥーホールド・メディエート・ストランド・スプレッシャー (HG-TMSD) という新しい合成サロゲットを導入する.
  • ダイナミックなDNA化学のツールボックスを拡張します
  • DNA鎖の移転プロセスを正確に制御する.

主な方法:

  • ゲストリンクされた入力配列とのバイオオートゴーナルクキュルビット[7]ウリル (CB[7]) の相互作用を利用した.
  • 入力シーケンス構造 (ヘッドグループ,リンク長) を変更することによって,HG-TMSDの調節が実証された.
  • HG-TMSDの微細と粗い調節のために競合する小分子ゲストを使用した.

主要な成果:

  • HG-TMSDの開発と特徴付けに成功しました.
  • 入力配列の修正により,鎖の移動運動の微調整が可能であることが示された.
  • 競合するゲストを使用してHG-TMSDの効果的な規制を証明した.
  • 酵素活性コントローラやマイクロRNA検出システムを含む機能的な装置にHG-TMSDを統合した.

結論:

  • HG-TMSDはダイナミックなDNA化学のための汎用性のあるプラットフォームを提供します.
  • このシステムは 複雑なDNAベースの装置に不可欠な 調整可能な制御を提供します
  • HG-TMSDはテラノスティクス,コンピューティング,バイオセンシングの 潜在的応用がある.