NINのようなタンパク質7の転写因子は植物性窒素センサーである
Kun-Hsiang Liu1,2,3, Menghong Liu1, Ziwei Lin1
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
Science (New York, N.Y.)
|September 22, 2022
まとめ
研究者は植物における 主要な窒素センサーを特定しました NINのようなタンパク質 (NLP) の転写因子の変異により,窒素反応は廃止され,植物成長と発達にとって重要な窒素センサーであるNLP7が明らかになった.
科学分野:
- 植物生物学
- 分子生物学
- 栄養素シグナリング
背景:
- 窒素は植物の成長に不可欠で 栄養素とシグナル分子の両方として作用します
- 植物には細胞内ナイトレートのレベルを感知するメカニズムがあり,それに従って代謝と発達過程を調節します.
研究 の 目的:
- 植物で窒素を検出する主要な分子センサーを特定する.
- 窒素が認識され,下流の反応を誘発するメカニズムを解明する.
主な方法:
- 変異とキメラの研究を含む,アラビドプシスのNINのようなタンパク質 (NLP) の転写因子の遺伝子分析.
- 窒素結合とタンパク質脱圧を分析する生化学的測定法
- 植物窒素動態の可視化のための遺伝的にコードされた光分裂バイオセンサ (mCitrine-NLP7) の開発と応用.
主要な成果:
- 7つのNLP遺伝子の変異により プライマリナイトレスの反応と 発達の可塑性が排除されました
- NLP7のアミノ端は,窒素の知覚とそれに続く減圧に関与する.
- NLP7の窒素センサドメインは,細菌のNreAとホモロジーを共有し,重要な残留物は,窒素によって引き起こされる転写制御,輸送,代謝,発達,およびバイオマスにとって重要である.
- mCitrine- NLP7バイオセンサを使用して単細胞窒素の動態を視覚化しました.
結論:
- NLP7は,陸上の植物の主要な窒素センサーとして特定されています.
- NLP7の保存された窒素センサー領域は,窒素シグナル伝達経路を媒介する上で重要な役割を果たします.
- NLP7の機能を理解することで 植物の栄養素感知と適応に関する洞察が得られます
さらに関連する動画
07:02TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
24.5K
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
14.1K
関連する概念動画
02:35Key Elements for Plant Nutrition
20.5K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
20.5K
01:25Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
01:56Riboswitches
8.4K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.4K
01:23Transcriptional Regulation: Riboswitches
100
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
100
01:22Inorganic Nitrogen Assimilation
81
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
81
02:00Photoreceptors and Plant Responses to Light
22.1K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
22.1K