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関連する概念動画

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

165
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
165
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

104
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
104
Homologous Recombination02:31

Homologous Recombination

50.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Updated: Aug 26, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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CRISPR 球状核酸

Chi Huang1, Zhenyu Han1, Michael Evangelopoulos2

  • 1Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208-3113, United States.

Journal of the American Chemical Society
|October 6, 2022
PubMed
まとめ
この要約は機械生成です。

研究者らは効率的なゲノム編集のために新しい CRISPR 球状核酸 (SNA) を開発した. これらのCas9 ProSNAは 厳格な方法を使わずに 細胞伝達と編集の効率を高め CRISPRの応用範囲を広げています

さらに関連する動画

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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関連する実験動画

Last Updated: Aug 26, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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科学分野:

  • バイオテクノロジー
  • 分子生物学
  • ナノテクノロジー

背景:

  • CRISPR/Cas9 ゲノム編集の効率は 細胞や組織に 部品を届けるという課題によって妨げられています
  • Spherical nucleic acids (SNAs) は細胞の吸収を高める可能性を秘めているが,遺伝子編集には適用されていない.

研究 の 目的:

  • 改良されたゲノム編集のための新しいクラスのCRISPR SNAを設計し評価する.
  • CRISPR-Cas9コンポーネントの細胞伝達と核の局所化を強化する.

主な方法:

  • 合成されたCas9 ProSNA:ガイドRNAをプリロードしたDNAの外部で密度が高いCas9タンパク質コア.
  • 組み込まれたGALAペプチドと核の局所化シグナルにより,エンドソームの脱出と核のターゲティングが改善されます.
  • プロテアゼ消化に対する安定性を評価し,複数の細胞系におけるゲノム編集効率を評価した.

主要な成果:

  • Cas9 ProSNAsは,電解または転移剤なしで細胞吸収が強化されたことが示されました.
  • 構造はプロテアゼ分解に対する安定性を示した.
  • 異なる細胞系で32%から47%のゲノム編集効率を達成しました.

結論:

  • 新型CRISPR SNA (Cas9 ProSNA) は,ゲノム編集における主要な配送障壁を克服しています.
  • これらのナノ構造は 細胞の吸収と編集の効率を大幅に改善します
  • この進歩はCRISPR-Cas9技術の応用と影響を拡大する可能性を秘めています