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関連する概念動画

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Restriction Enzymes01:11

Restriction Enzymes

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Stereoisomerism02:52

Stereoisomerism

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Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
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関連する実験動画

Updated: Aug 26, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

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配列で定義されたオリゴマーの同位体比エンコーディング

Márton Zwillinger1,2, Lucile Fischer3, Gergő Sályi1

  • 1Servier Research Institute of Medicinal Chemistry, H-1031 Budapest, Hungary.

Journal of the American Chemical Society
|October 7, 2022
PubMed
まとめ

科学者は,独特の分子指紋を作成するために,デュテリウムラベル付きのモノマーを使用して,新しい同位体比エンコーディング方法を開発しました. この技術は,複雑な分子構造を区別する質量スペクトロメトリーによる高精度情報読み取りを可能にします.

さらに関連する動画

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

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関連する実験動画

Last Updated: Aug 26, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
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科学分野:

  • 分子生物学
  • 分析化学
  • バイオテクノロジー

背景:

  • 分子情報保存は,しばしばオーダーされたモノマー配列と質量スペクトロメトリーに依存する.
  • 現在の方法は,複雑な断片化または分子混合をデータ取得に含みます.

研究 の 目的:

  • 分子情報保存のための代替アイソトープ比エンコーディング戦略を導入する.
  • オリゴマーの独特の同位体分布パターンを基に区別する能力を実証する.

主な方法:

  • デュテリウムラベルを貼ったモノメアを使って 何百ものユニークなオリゴメアを合成する.
  • 異なる同位体分布パターンを認識するために質量スペクトロメトリーを使用します.
  • デュテラートされた構成要素を用いた4つの異なるモノマーからなる256個のテトラメールを区別する.

主要な成果:

  • アイソトープ比パターンを用いて高精度でオリゴーマーに情報をエンコードし,解読しました.
  • モノ-, ディ-, トリ-, テトラデュテラートモノマーから派生した質量指紋に基づいてテトラーマーを区別する能力を示した.
  • 実験誤差に対する方法の堅強さと,標準的な質量スペクトロメトリ機器との互換性を確認した.

結論:

  • 同位体比エンコーディングは,同位体分布を通じて情報を分子化合物に書き込むための直接的な方法を提供します.
  • このアプローチは,追加のタグ付けや分子混合物の使用の必要性を回避します.
  • この技術は分子アイデンティティのエンコーディングと 先進的なデータストレージでの応用の可能性を持っています