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Cell Migration01:09

Cell Migration

17.1K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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G Protein-coupled Receptors01:15

G Protein-coupled Receptors

12.9K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
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Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

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Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
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G-protein Coupled Receptors01:21

G-protein Coupled Receptors

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Updated: Aug 25, 2025

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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GPC3-Unc5受容体複合体の構造と細胞移動における役割

Onno Akkermans1, Céline Delloye-Bourgeois2, Claudia Peregrina3

  • 1Department of Biochemistry, University of Oxford, Oxford, UK.

Cell
|October 14, 2022
PubMed
まとめ

この研究は,グリピカン-3 (GPC3) に結合するアンコーディネート-5受容体D (Unc5D) の構造を明らかにし,その相互作用が神経細胞の移動と癌細胞の拡散をどのように導いているかを明らかにした.

キーワード:
GPC3 についてUNC5AUNC5BUNC5CUNC5DUnc5 についてセルガイド細胞の移動皮質の発達クリスタルグラフィーグリピカン-3ナノボディ神経芽細胞腫ストライプアッセイ構造生物学表面プラズモンの共鳴調整されていない-5

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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
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関連する実験動画

Last Updated: Aug 25, 2025

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

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Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
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Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

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科学分野:

  • 神経科学
  • 構造生物学
  • 癌 生物学

背景:

  • 神経の移動は 細胞表面の誘導受容体を含む脳の発達に不可欠です
  • 癌細胞はこれらの発達メカニズムを転移のために利用します

研究 の 目的:

  • Uncoordinated-5受容体D (Unc5D) とグリピカン-3 (GPC3) の相互作用の構造的基礎を解明する.
  • Unc5D-GPC3複合体の発達と癌における細胞誘導における役割を理解する.

主な方法:

  • オクタメリック複合体の構造を決定するX線結晶学
  • 分子ダイナミクスシミュレーションと質量スペクトロメトリを検証する.
  • マウスおよび異種移植モデルにおける構造ベースの変異性およびナノボディ測定.

主要な成果:

  • 結晶構造は,中央のグリカン-グリカン相互作用を持つオクタメリックUnc5D-GPC3複合体を明らかにした.
  • Unc5D媒介のGPC3とC-マノシル化トリプトファンからのN結合グリカン.
  • Unc5D- GPC3の相互作用は,ピラミッド神経細胞の移動と神経芽細胞の拡散を誘導することが示された.

結論:

  • Unc5D- GPC3相互作用による細胞誘導の保存された構造的メカニズムが実証されました.
  • 微妙にバランスのとれたUnc5D- GPC3結合は,発達と疾患の両方で細胞移動の調節に不可欠です.