関連する実験動画

Updated: Aug 23, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

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対象外活動のための構造的基礎

Martin Pacesa1, Chun-Han Lin2, Antoine Cléry3

  • 1Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Cell
|October 28, 2022
PubMed
まとめ

CRISPR-Cas9によるゲノム編集は 意図しないDNA配列を標的とし 安全性の懸念を提起します 構造分析により,非正規の塩基配列とデレーションの収納により,標的外結合が可能になり,ガイドRNAの設計が改善された.

キーワード:
CRISPRについてカス9X線結晶学ベースペアリングゲノム編集ガイドRNA不一致核酵素ターゲット外

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