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関連する概念動画

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

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Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
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Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

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Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
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PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

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The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
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Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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Updated: Aug 23, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

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多相凝結はヒッポ経路の活性化を媒介する.

Li Wang1, Kyungsuk Choi1, Ting Su1

  • 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Cell
|November 1, 2022
PubMed
まとめ

科学者は生物分子の凝縮物を 溶かさずに制御する 新しい方法を発見しました これはヒッポシグナル伝達経路のアップストリームレギュレータが 細胞機能を調節するために融合する 明確なコンデンサートを形成することを含みます

キーワード:
アモット (AMOT)海馬の経路キブラSTRIPAK (ストライパック)YAP についてバイオ分子凝縮物細胞同士の接触細胞骨格多相オスモティック・ストレス

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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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Culturing and Manipulation of O9-1 Neural Crest Cells
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Culturing and Manipulation of O9-1 Neural Crest Cells

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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Culturing and Manipulation of O9-1 Neural Crest Cells
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Culturing and Manipulation of O9-1 Neural Crest Cells

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科学分野:

  • 細胞生物学
  • 生物化学
  • 分子生物学
  • 信号変換

背景:

  • 生物分子凝縮物の機能は溶解によってしばしば制限される.
  • 溶解せずにコンデンサートの活動を抑制するメカニズムは,ほとんど不明のままです.
  • 細胞の成長と臓器の大きさに 重要な役割を果たします

研究 の 目的:

  • 生物分子凝縮体の機能が溶解せずに抑制できるかどうかを調査する.
  • ヒッポシグナル伝達経路の活動を調節する上流調節体の役割を明らかにする.
  • 生物学的システムにおける多相組織の原理を理解する.

主な方法:

  • SLMAP,AMOT,KIBRAによって形成された凝縮物の形成と相互作用を調査した.
  • コンデンサトの凝結とSTRIPAK複合体の機能に対するその効果を観察する技術を用いた.
  • 異なる細胞条件下でヒッポシグナル伝達経路の上流調節を分析した.

主要な成果:

  • ヒッポ経路の上流のレギュレータは,機能的に敵対するコンデンサートを形成します.
  • SLMAPはヒッポの不活性化コンデンサを形成し,AMOTとKIBRAはヒッポの活性化コンデンサを形成する.
  • これらの相反するコンデンサートが共通の相に凝結することで,STRIPAK複合体の機能を抑制し,溶解せずに経路の活動を抑制します.

結論:

  • 溶解せずに生物分子凝縮物の活動を調節するための新しいパラダイムが提示されています.
  • 機能的に敵対するコンデンサートを含む多相組織は,細胞プロセスを微調整するメカニズムを提供します.
  • この研究は,ヒッポシグナル伝達経路とコンデンサート行動の上流調節に関する洞察を提供します.