Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Protein Folding01:25

Protein Folding

8.3K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
8.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.0K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.0K
Mechanical Protein Function01:58

Mechanical Protein Function

2.1K
2.1K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.8K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.9K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Development of a glutamine-responsive MRI contrast agent.

Chemical science·2025
Same author

Mechanistic Interrogation of a PQQ and Rare Earth-Dependent Artificial Metalloenzyme.

Journal of the American Chemical Society·2025
Same author

Facile Generation of Cyanoselenocysteine as a Vibrational Label for Measuring Protein Dynamics on Longer Time Scales by 2D IR Spectroscopy.

Analytical chemistry·2025
Same author

Chlorophyllase from <i>Arabidopsis thaliana</i> Reveals an Emerging Model for Controlling Chlorophyll Hydrolysis.

ACS bio & med chem Au·2024
Same author

Conformational control over proton-coupled electron transfer in metalloenzymes.

Nature reviews. Chemistry·2024
Same author

Structure-driven development of a biomimetic rare earth artificial metalloprotein.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same journal

Radical Cascades on Seawater Microdroplets Drive Atmospheric Mercury Oxidation.

Journal of the American Chemical Society·2026
Same journal

Superior Selective and Fast NH<sub>3</sub> Adsorption of Soft Porous MOF/Ionic Liquid Composites with Ordering Phase Transitions.

Journal of the American Chemical Society·2026
Same journal

Systematic Catalyst Variation for Improved Stereoselective Epoxide Polymerization: Subtle Modifications Resulting in Superior Efficiency.

Journal of the American Chemical Society·2026
Same journal

Deciphering the Halide Chemistry of Cl<sup>-</sup> and Br<sup>-</sup> in Enhancing Kinetics of Mg Plating/Stripping.

Journal of the American Chemical Society·2026
Same journal

Electrosynthesis of C<sub>6</sub> Chemicals by Propylene Oxidative Coupling on Au Surface.

Journal of the American Chemical Society·2026
Same journal

Statistical AI Enables Precise Screening of Multielement Catalysts.

Journal of the American Chemical Society·2026
関連記事をすべて見る

関連する実験動画

Updated: Aug 21, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K

形状的に切り替える人工メタロタンパク質の設計

Saman Fatima1, David G Boggs2, Noor Ali3

  • 1Department of Chemistry, University of Illinois Urbana-Champaign, 600 S. Mathews Avenue, Urbana, Illinois61801, United States.

Journal of the American Chemical Society
|November 15, 2022
PubMed
まとめ
この要約は機械生成です。

研究者らは,リガンド結合時に形を変えた切替可能な人工メタロタンパク質 (swArM) を開発した. このプラットフォームでは,天然の金属酵素を模倣して,タンパク質のダイナミクスとメタルコファクターの活性がどのように相互に結びついているかを研究しています.

さらに関連する動画

Synthesis of a Water-soluble Metal&#8211;Organic Complex Array
06:40

Synthesis of a Water-soluble Metal–Organic Complex Array

Published on: October 8, 2016

9.9K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.5K

関連する実験動画

Last Updated: Aug 21, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K
Synthesis of a Water-soluble Metal&#8211;Organic Complex Array
06:40

Synthesis of a Water-soluble Metal–Organic Complex Array

Published on: October 8, 2016

9.9K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.5K

科学分野:

  • バイオ有機化学
  • タンパク質工学
  • 生物物理化学

背景:

  • メタロ酵素は,タンパク質の構造とメタロコファクターの活性を結びつける,構造変化に依存する.
  • 現存する人工メタロタンパク質 (ArM) は,多くの場合,ダイナミックな柔軟性を欠き,自然のシステムをモデル化する能力を制限しています.
  • メタロコファクターの反応性を調節するアロステリーの役割を理解することは,バイオインスピレーションによる触媒設計に不可欠です.

研究 の 目的:

  • 大規模な構造変化を経験する形状的に切り替え可能な人工メタロタンパク質 (swArM) を設計する.
  • タンパク質の構造動態とメタルコファクターの電子構造と反応性の相互作用を調査する.
  • メタロコファクター機能のアロステリック調節の研究のためのプラットフォームを確立する.

主な方法:

  • コバルト ((II) ビス ((dimethylglyoxime) (Co ((dmgH) 2 ((X)) メタロコファクターをE. coliのグルタミン結合タンパク質 (GlnBP) に具体的組み込む.
  • 特徴付けのためのスペクトロスコーピック技術 (UV-vis,光,CD,IR) と質量スペクトロメトリ.
  • 構造的決定のためのX線結晶学と結合研究のための同熱定位カロメトリー.

主要な成果:

  • GlnBP内のメタロコファクターのサイト固有のステキオメトリックC-Sシステイン結合でSWARMを成功裏に設計した.
  • アロステリックグルタミン結合がタンパク質の構造の変化を誘発することを実証した.
  • タンパク質環境がCo-S結合を安定させ,構造の変化が解離率を調節することを示した.

結論:

  • アロステリー駆動メタルコファクター調節の調査を可能にする新しい swArM プラットフォームを開発しました.
  • 人工系におけるタンパク質の構成動態とメタルコファクターの反応性との直接的な関連を確立した.
  • 自然な金属酵素の仕組みを模倣し理解するための強力なツールです.