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Updated: Aug 20, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

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Fe (II) /α-ケトグルタレット依存酸素酵素TqaLのステレオ選択性と基板特異性

Hui Tao1, Richiro Ushimaru1,2,3, Takayoshi Awakawa1,2,4

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.

Journal of the American Chemical Society
|November 17, 2022
PubMed
まとめ

TqaLのような非ヘム鉄酵素は,ステレオ化学的に複雑なプロセスを経てアミノ酸からアジリジンを形成する. 変異は反応結果と基質特異性を制御する重要な残基を明らかにする.

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Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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The carbonyl center is...
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