ホルモン受容体に対する病原体干渉のNLR監視は免疫を誘発する
Jing Chen1, Yanxiao Zhao1, Xuanjie Luo1
1Key Laboratory of Plant Immunity, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
Nature
|December 14, 2022
まとめ
植物 NLR タンパク質は,植物ホルモンシグナル伝達を妨げる病原体エフェクターを検出します. 胡のTswタンパク質はTSWV NSsエフェクタを認識し,TCP21を標的としてホルモン受容体を阻害し,それによって植物免疫を活性化します.
科学分野:
- 植物免疫
- フィトホルモンシグナル伝達
- 分子植物病原体相互作用
背景:
- 植物ホルモンのシグナル伝達は 病原体に対する植物の防御に不可欠です
- 病原体はフィトホルモン経路を操作して 毒性を促進します
- ホルモンのシグナル伝達に対する病原体の監視は,十分に理解されていません.
研究 の 目的:
- 植物内在の免疫系が 植物ホルモンのシグナル伝達に 病原体の干渉を監視する方法を 研究する.
- 胡 NLR Tsw が TSWV エフェクター NSs を認識するメカニズムを明らかにする.
- 植物ホルモンの信号伝達経路を NS がどう操作するかを理解するためです
主な方法:
- Tsw LRRドメインとホルモン受容体を比較するための構造モデリング.
- タンパク質の相互作用を研究するための共免疫プレシピテーションアッセイ
- ウイルスが誘発した遺伝子静止は,TCP21の役割を評価する.
主要な成果:
- TSWVエフェクターNSsは,抑制剤TCP21を介して植物ホルモン受容体 (COI1,TIR1,MAX2) と相互作用する.
- NSsはNSs- TCP21受容体の相互作用を強化し,ホルモン媒介の免疫を阻害する.
- NLR TswはTCP21と相互作用し,この相互作用はNSによって強化され,防御が活性化されます.
結論:
- 病原菌エフェクターは,TCP21のような保存された抑制剤を標的とし,植物ホルモンシグナル伝達を妨害し,毒性を促進することができます.
- 植物のNLRタンパク質は 効果因子による干渉を検知し 免疫を活性化するために進化した.
- この研究は,植物ホルモン経路の病原体操作に対する 植物免疫監視の新しいメカニズムを明らかにしています
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