5S遺伝子の内部制御領域は,溶液中のB型であり,TFIIIAIAとの複合体である
J M Gottesfeld1, J Blanco, L L Tennant
1Department of Molecular Biology, Research Institute of Scripps, La Jolla, California 92037.
Nature
|October 1, 1987
まとめ
クセノプス5SRNA遺伝子について
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- 遺伝学 遺伝学とは
背景:
- クセノプス5SRNA遺伝子の内部制御領域は,転写に不可欠である.
- 以前の研究では,配列分析と短いDNA断片に基づいて,この領域のためのA型DNA構造を提案しました.
- この領域は,亜鉛結合タンパク質である転写因子IIIA (TFIIIA) に結合する.
研究 の 目的:
- 溶液中のXenopus 5S RNAの内部制御領域のDNA構造を調査する.
- 前述の通り,DNAがA型構造を採用するかどうかを判断する.
- TFIIIAがDNAに結合した時の構造変化を検証する.
主な方法:
- 円形二重化 (CD) スペクトロスコーピーは,TFIIIA結合部位に対応する合成54bpデオキシオリゴヌクレオチドを分析するために使用されました.
- DNAのCDスペクトルは,既知のB型DNAスペクトルと比較した.
- DNA-TFIIIA複合体のCDスペクトルを分析し,構造的変化を検出しました.
主要な成果:
- 合成54bpデオキシオリゴヌクレオチドのCDスペクトルは,B型DNAのCDスペクトルと似ていた.
- DNA-TFIIIA複合体は,変化のないDNACD成分を示した.
- TFIIIA結合の際には,DNA構造の重大な変化は見られなかった.
結論:
- Xenopus 5S RNA遺伝子の内部制御領域は,溶液中のB型DNA構成に存在する可能性が高い.
- TFIIIAの結合は,DNAの重要な構造的変化を誘導しない.
- この発見は,機能的に重要なこの領域におけるA型DNA構造に関する以前の仮説に異議を唱える.
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