関連する実験動画
Updated: Aug 17, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
網膜芽細胞腫の感受性遺伝子は,DNA結合活性に関連した核フォスフォタンパク質をコードする
1Department of Pathology, University of California San Diego, School of Medicine, La Jolla 92093.
Nature
|October 15, 1987
まとめ
研究者は,遺伝性網膜芽細胞腫の重要な要因であるRBタンパク質を特定しました. このがん抑制タンパク質は腫瘍に存在しないため,遺伝子を調節し,がんを予防する役割があることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- がん研究 がん研究
背景:
- 遺伝性網膜芽細胞腫は,RB遺伝子と関連しています.
- RB遺伝子の完全無効化は,腫瘍形成に必要である.
- RB遺伝子は,がん抑制剤として作用し,腫瘍遺伝子の機能に反する.
研究 の 目的:
- RB遺伝子のタンパク質産物を特定し,特徴づけること.
- RBタンパク質の機能と局所を調査する.
主な方法:
- 分子遺伝技術とRB cDNAの配列分析.
- トライペ-RB融合タンパク質に対するウサギの抗菌剤の調製.
- 免疫プレシピテーション,代謝ラベリング (32P-リン酸),生化学分化,免疫光研究.
主要な成果:
- RBタンパク質 (110,000~114,000 Mr) は,特定の抗菌剤を用いて免疫圧縮された.
- RBタンパク質は正常な細胞系に存在するが,網膜芽細胞腫細胞系には存在しない.
- RBタンパク質は,DNA結合活性を持つ核フォスフォタンパク質である.
結論:
- RB遺伝子製品は,DNA結合能力を有する核フォスフォタンパク質である.
- レチノブラストーマ細胞におけるRBタンパク質の欠如は,腫瘍抑制剤としての役割を示唆している.
- RBタンパク質は,おそらく他の遺伝子を調節し,細胞の成長制御に貢献します.
関連する概念動画
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
01:45Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...