メタトランスクリプトーム を 採掘 する こと に よっ て,広大 な ウイルス の よう な 円形 の RNA が 発見 さ れ ます
Benjamin D Lee1, Uri Neri2, Simon Roux3
1National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA; Nuffield Department of Medicine, University of Oxford, Oxford OX3 7BN, UK.
Cell
|January 25, 2023
まとめ
研究者は,計算パイプラインを使用して数千の新しいビロイドのような円形RNA (cccRNA) を発見し,これらの最小の複製体の既知の多様性を大幅に拡大し,これまで理解していたよりも広いホスト範囲を示唆しました.
科学分野:
- ウイルス学
- コンピュータ生物学
- ゲノミクス
背景:
- ウイルス型およびウイルス型型の円形RNA (cccRNA) は,タンパク質をコードする遺伝子が欠けている最小の複製体である.
- その複製は宿主細胞の仕組みに依存している.
- これらのエージェントの完全な範囲と多様性は,ほとんど未知のままです.
研究 の 目的:
- ウイルス型cccRNAを特定するための計算パイプラインを開発し,適用する.
- 様々なトランスクリプトームにおけるこれらのエージェントの多様性と流行を評価する.
- ウイルスのような新種の元素と 宿主を発見するためです
主な方法:
- cccRNAを特定するための計算パイプラインの開発.
- パイプラインを5,131個のメタトランスクリプトームと1,344個の植物トランスクリプトームに適用する.
- 分類と特徴づけのための特定されたcccRNAのバイオ情報分析.
主要な成果:
- 4,409種のクラスターに 11,378のビロイド型cccRNAが特定され,以前の発見の5倍となった.
- 多様な疑似ウイルス,衛星RNA,レトロジーム,およびリボジーム型ウイルスの発見.
- 様々なccRNA型における自己分裂リボ酵素の検出と,プロカリオトの複製を示唆する証拠.
結論:
- ウイルスのようなcccRNAは,これまで認識されていたよりも多様で広く存在しています.
- これらのエージェントは,潜在的にプロカリオットを含むより広いホスト範囲を有しています.
- これらの発見は,様々な生態系における最小複製体の役割と影響を再評価することを必要としています.
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