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関連する概念動画

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

99
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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関連する実験動画

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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条件付きRNAの調節と編集のための分割CRISPR/Cas13bシステム

Ying Xu1, Na Tian1, Huaxia Shi1

  • 1Department of Chemistry, Case Western Reserve University, 2080 Adelbert Road, Cleveland, Ohio 44106, United States.

Journal of the American Chemical Society
|February 22, 2023
PubMed
まとめ
この要約は機械生成です。

研究者は,アブシシス酸 (ABA) と光で制御可能な新しいCRISPR/Cas13bシステムを開発しました. このシステムはRNAのレベルと変化を正確に制御し,RNAの研究ツールを進歩させています.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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関連する実験動画

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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科学分野:

  • 分子生物学
  • バイオテクノロジー
  • RNA 生物学

背景:

  • CRISPR/Cas13bシステムは RNAの研究の強力なツールです
  • Cas13bの活性に対する正確な制御は,RNAの機能を理解するために不可欠です.
  • 既存のツールは ネイティブRNAの活動に 干渉することが多いのです

研究 の 目的:

  • 条件付きRNA調節のための分割Cas13bシステムを設計する.
  • 精密なRNA操作のためのABA誘導システムを開発する.
  • RNA 改変堆積の光ベースの制御を探求する.

主な方法:

  • アブシシ酸 (ABA) によって活性化されるCRISPR/Cas13bの分割システムを設計する.
  • m6Aの堆積のために,ABA誘導可能な分割dCas13bを開発する.
  • 光媒介制御のための光活性化ABA誘導体を利用する.
  • 投与量と時間によるRNAの低下を証明した.

主要な成果:

  • ABAを用いたCas13b/dCas13bの条件付き活性化と非活性化
  • m6Aの特定の部位での一時的に制御された堆積.
  • 分割されたCas13b/dCas13bシステムの光誘導調節
  • 投与量と時間による内生RNAの低下.

結論:

  • 開発されたCas13b/dCas13bスプリントシステムは,RNA操作に対する正確な時間的および条件制御を提供します.
  • これらのプラットフォームは,CRISPRとRNAの規制ツールキットをネイティブの細胞環境に拡張します.
  • システムは内生RNAの機能的障害を最小限に抑え,高度なRNA研究を容易にする.