c-myc遺伝子は, RNAポリメラーゼIIとIIIのプロモーターを重ね合わせたものをコードする

J Chung¹, D J Sussman, R Zeller

¹Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts 02115.

Cell

|December 24, 1987

まとめ

c-myc遺伝子は,c-myc遺伝子と呼ばれるものです.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
ゲノミクスゲノミクスとは

背景:

c-myc遺伝子の最初のエクソンは,頻繁な転写と保存を含むユニークな特徴を示しています.
これらの性質は,単純なタンパク質のコーディングを超えて,規制的な役割を果たすことを示唆しています.

研究の目的:

c-myc遺伝子の最初のエクソンの異常な性質を調査するために.
高い転写率と規制の可能性の根本的なメカニズムを解明する.

主な方法:

浄化されたRNAポリメラーゼIIおよびIII (pol IIおよびIII) を使用したインビトロ転写アッセイ.
主要c-mycプロモーター (P2) での転写開始と終了の分析.
最初のエクソンの3'端のポリメラーゼの行動を調査する.

主要な成果:

主要なc-mycプロモーター (P2) は二機能であり,pol IIとpol IIIの両方のイニシアチブをサポートします.
Pol IIIは最初のエクソンの3'端の近くでブロックされ,Pol IIは部分的にのみブロックされます.
異なるポリメラーゼ活性によって,新しい遺伝子調節メカニズムが示唆されている.

結論:

c-mycプロモーターの二機能性と微分ポリメラーゼの停止は,c-myc遺伝子発現を調節するメカニズムを提供します.
この重複した転写活動により,ポリメラーゼ系が他のポリメラーゼ系に影響を与え,遺伝子制御に寄与する可能性があります.

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